花魔芋抗软腐病植株的鉴定及其抗性机理的初步研究  

Characterization of Soft-rot-resistant Amorphophallus konjac and Preliminary Analysis of the Resistance Mechanism

作　　者：雷珍珍[1] 叶晶龙[1] 程海丽[1] 陈云[1] 望彗星[1] 许克静[1] 乐超银[1] 

机构地区：[1]三峡大学生物技术研究中心,宜昌443002

出　　处：《植物学报》2013年第3期295-302,共8页Chinese Bulletin of Botany

基　　金：国家自然科学基金(No.30871579);湖北省自然科学基金(No.2011CDB184)

摘　　要：通过筛选获得了对魔芋(Amorphophallus konjac)软腐病具有较强抗性的花魔芋抗病植株,经核型分析发现,其染色体数目与普通植株一致,均为2n=26。抗病实验结果表明,该抗病植株对魔芋软腐病的抗性较普通植株强。采用酶联免疫吸附法,测定了软腐病病原菌接种30小时内的抗病与普通植株叶片中内源植物激素(SA与JA)的含量变化。结果显示,抗病和普通植株叶片中SA和JA含量变化的总体趋势明显不同。主要表现在,抗病和普通植株叶片中SA和JA积累的时间不同;此外,积累的量也有明显差异。推测魔芋抗病植株可能存在与目前大多数植株不同的抗病机制。Amorphophallus konjac plants with strong resistance to soft-rot disease were identified from the A. konjac field in Changyang, Yichang. Karyotype analysis showed that the number of chromosomes in resistant plants were consistent with that in common A. konjac plants （susceptible）, and both were 2n=26. Resistant plants were compared with suscep- tible A. konjac plants for disease resistance. Soft-rot-resistant plants showed stronger resistance against Erwinia caroto- vora var. carotovora than susceptible plants did. ELISA revealed hormone levels induced within 30 h after infection with E. carotovora. However, susceptible plants showed higher levels of jasmonate and salicylic acid than resistant plants did. These results imply a different disease resistance mechanism in resistant A. konjac plants, which can be examined by further studies.

关 键 词：软腐病 魔芋 抗病 水杨酸 茉莉酸 

分 类 号：S632.9[农业科学—蔬菜学]