人组织激肽释放酶对大鼠坐骨神经损伤的修复作用  

Repair effect of human tissue kallikrein on sciatic nerve injury of rats

作　　者：耿武军[1] 刘珺[2] 唐红丽[1] 莫云长[1] 王丹丹[1] 王均炉[1] 

机构地区：[1]温州医学院附属第一医院麻醉科 [2]安徽医科大学附属第三医院麻醉科

出　　处：《中国临床药理学与治疗学》2013年第6期610-613,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：温州市科技局项目(Y20100217)

摘　　要：目的:观察人组织激肽释放酶(HTK)对大鼠坐骨神经损伤的修复效应。方法:选取36只雄性SD大鼠,重量在180～220g之间,分离坐骨神经,造成坐骨神经挤压伤模型,然后随机均分成3组:对照组,自尾静脉每日注射生理盐水2mL;甲强龙组(SM组),自尾静脉每日注射甲强龙30mg/kg(稀释至2mL);人组织激肽释放酶组(HTK组),自尾静脉每日注射HTK 17.5×10-3PNAU/kg(稀释至2mL)治疗。在术前及术后第1、3、5、7、9、11、13天各时间点测定坐骨神经功能指数(SFI),术后第14天取出坐骨神经干,测定动作电位传导速度(NCV)。结果:三组大鼠SFI值在术后第1、3天均为-100左右。自第3天开始,HTK组和SM组SFI恢复的情况要优于对照组,有统计学意义(P<0.05)。在术后第14天HTK组和激素组的NCV值高于对照组,差异有统计学意义(P<0.05)。结论:大鼠经尾静脉注射HTK,可促进坐骨神经损伤修复,神经功能恢复快。【Abstract】 Objective Studing the repairaton of human tissue kallikrein（HTK）on sciatic nervous of rars. Methods 36 adult male SD rats weighted 180-220g were randomly divided into 3 groups：The control group was just injected with 2ml salt water by caudal vain every day . The SM group was injected with methylprednisolone 30mg/Kg（2ml）by caudal vain every day . The HTK group was injected with human tissue kallikrein 17.5×10^-3 PNAU/Kg（2ml）by caudal vain every day. Calculating sciatic functional index （SFI） at the day 1,3,5,7,9,11 and 13 after operation in all rats. Observating the nerve conduction velocity of the sciatic nerve at the 14th day postoperation. Results All rats showed -100 at day 1and 3 . The HTK group and the SM group showed a statistically significant improvement over the control group from the 3th day postoperation. The NCV of the HTK group and the SM group were greater than the control group （P〈0.05）. Conclusion The repairaton of human tissue kallikrein（HTK）on sciatic nervous of rars was effective.

关 键 词：人组织激肽释放酶 坐骨神经损伤 激素 修复作用 

分 类 号：R965.2[医药卫生—药理学]