孕鼠感染犬弓首线虫后对仔鼠行为及脑组织的影响  

Offspring Behavioral and Brain Pathological Changes Caused by Toxocara canis Infection to Pregnant Mice

作　　者：陈琨[1,2] 令狐克燕 李建华[1] 李金福[1] 

机构地区：[1]贵阳医学院寄生虫学教研室 [2]贵州省人民医院中心实验室

出　　处：《贵阳医学院学报》2013年第3期243-245,共3页Journal of Guiyang Medical College

基　　金：黔科合LG字[2011]009号;黔科平台[2010]4005;[2010]筑科技合同字3-1号;黔科合sy[2010]3132号

摘　　要：目的:孕鼠妊娠早期感染犬弓首线虫对仔鼠行为及脑组织的影响。方法:取犬弓首线虫子宫段虫卵培养至感染期,不同剂量感染受孕3 d的雌鼠,将仔鼠随机分6组,观察爬行能力、抓握能力等行为学情况,用切片法及稀盐酸幼虫分离法观察犬弓首线虫在仔鼠脑内分布。结果:从新生仔鼠脑内分离出幼虫,脑内幼虫的数量与孕期感染剂量呈正相关;各组仔鼠行为学检测结果差异具有统计学意义(P<0.05),与脑内分离出的幼虫数量呈负相关;仔鼠脑内有少量胶质细胞增生和脑细胞变性,虫体周围脑组织炎症反应不明显。结论:孕鼠妊娠早期感染犬弓首线虫会导致仔鼠行为学改变,感染剂量与仔鼠后天产生的保护性免疫记忆有相关性,脑内病理改变与直接感染一致。Objective： To investigate if Toxocara cauls infection during early pregnancy could cause behavior and brain pathological changes of mouse offspring. Methods： Three-day-pregnant female mice were infected with different doses of Toxocara canis eggs at infective phase. The offsprings were divided into six groups and their behavior （maze, grip, balance） was observed. Sectioning method and dilute hydrochlonic acid separation method were adopted to observe larval distribution in the brain tissue and brain pathological changes. Results： Behavioral changes of offsprings were significantly dif- ferent among groups （P 〈 0.05 ） and were positively correlated with the number of larvae isolated from brain tissue. Pathological changes were found in offspring brain. Conclusions： Toxocara canis infec- tion during early pregnancy may affect offspring＇s behavior. Infection during pregnancy caused brain tis- sue pathological changes are accordance with that of direct infection.

关 键 词：犬弓首线虫 妊娠母鼠 仔鼠 Barnes迷宫 行为改变 病理学 稀盐酸幼虫分离法 

分 类 号：R382.5[医药卫生—医学寄生虫学]