梓醇对APP/PS1双转基因小鼠焦虑行为和脑内神经元的影响  

Effects of catalpol on anxious behaviors and neurons in APP/PS1 double transgenic mice

作　　者：宋冲[1] 楚亚楠[1] 贺桂琼[2] 刘刚[1] 王凌晞[1] 周泽芬[1] 姚秋会[1] 

机构地区：[1]重庆医科大学重庆市神经生物学重点实验室,重庆400016 [2]重庆医科大学基础医学院解剖教研室,重庆400016

出　　处：《重庆医科大学学报》2013年第6期598-601,共4页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(编号:30970986);教育部"新世纪优秀人才"支持计划资助项目(编号:NCET-11-1084);重庆市首届优秀人才资助计划资助项目[编号:渝教人(2009)2号];留学人员科技活动项目择优资助项目[编号:渝人社办〔2011〕235号]

摘　　要：目的:观察梓醇对APP/PS1双转基因小鼠焦虑行为和脑内神经元的影响。方法:将3月龄的APP/PS1双转基因小鼠按随机数字表法分为梓醇治疗组和生理盐水对照组,每组10只,用梓醇[(5 mg/(kg.d)]和等量生理盐水腹腔注射阿尔茨海默病(Alzheimer’s disease,AD)模型小鼠3周,应用高架十字迷宫检测两组小鼠的焦虑情绪的变化;应用免疫组织化学染色和Western blot检测脑内神经元及突触相关蛋白的表达变化。结果:与生理盐水对照组比较,梓醇治疗组小鼠进入开放臂的次数(P=0.000 2,t=4.742)和时间(P=0.001,t=3.936)显著增加,神经元数量(P=0.028 4,t=2.872)及突触蛋白表达(P=0.002,t=5.265)明显增多。结论:梓醇治疗能显著缓解AD模型小鼠的焦虑情绪,这种改善作用可能是通过改善神经元及突触的丢失来实现的。Objective：To research whether catalpol affect anxious behaviors and neurons in APP/PS1 double transgenic mice.Methods：Three-month old APP/PS1 double transgenic mice were randomly divided into catalpol-treated and saline-treated groups（n=10）.5 mg/（kg·d） of catalpol and the same amount of saline were peritoneally injected into Alzheimer＇s disease（AD） model mice for 3 weeks.Elevated plus-maze test was performed to test autono-mous behaviors of AD model mice;immunohistochemical staining and Western blot were used to detect number of neurons and expressions of NeuN and PSD95 in the brain of mice.Results：Compared with those of saline-treated AD model mice,open arm entries（P=0.000 2,t=4.742） and open arm time（P=0.001,t=3.936） catalpol-treated AD mice were significantly decreased（P=0.028 4,t=2.872）,number of neurons were significantly increased（P=0.028 4,t=2.872） and expressions PSD95 were also increased（P=0.002,t=5.265）.Conclusions：Catalpol can significantly reduce anxious behaviors,which shows that catalpol may exert its neuroprotection through suppressing the wide loss of neurons and synapses in the brain of APP/PS1 double transgenic mice.

关 键 词：梓醇 阿尔茨海默病 焦虑行为 神经元 突触 

分 类 号：R285.5[医药卫生—中药学]