EGFR/STAT信号通路相关蛋白在特发性肺纤维化中的表达及临床意义  

Expression and clinical significance of the EGFR/STAT signal pathway in idiopathic pulmonary fibrosis

作　　者：李龙[1] 杨兰生[2] 李慧[3] 胡静怡[3] 唐发兵[2] 余勤[1] 

机构地区：[1]兰州大学第一医院呼吸科,甘肃兰州730000 [2]兰州大学第一医院病理科,甘肃兰州730000 [3]兰州大学医学院,甘肃兰州730000

出　　处：《西安交通大学学报（医学版）》2013年第4期500-502,553,共4页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的通过检测特发性肺纤维化(IPF)患者肺组织中表皮生长因子受体(EGFR)、细胞外调节蛋白酶(ERK1/2)和信号传导转录激活因子(STAT1)的表达,探讨与IPF临床特征及预后的关系。方法选择15例IPF患者及10例正常肺组织标本,经石蜡包埋切片后,行免疫组化检测,并与肺功能等检测指标结果进行分析。结果 IPF肺组织中EGFR和STAT1呈阳性表达分别为11/15例、10/15例,且两者表达与IPF患者肺总量、弥散功能及PaO2水平呈显著正相关(P<0.05),与IPF病理特征和高分辨率胸部CT影像特征之间无显著相关性;而在10例对照组肺组织中EGFR和STAT1均无明显阳性表达;IPF肺组织中RAS/MAPK信号转导分子ERK1/2表达水平较对照组肺组织无明显增强(P>0.05)。结论 EGFR/STAT1信号通路活化与IPF的发生、发展密切相关,可作为诊断和评估IPF进展的有价值的指标。Objective To investigate the expressions of epithelial growth factor receptor（EGFR）,extracellular signal-regulated kinase（ERK1/2） and signal transducer and activator of transcription（STAT1） in patients with idiopathic pulmonary fibrosis（IPF） in order to explore their relationship with the clinical characteristics and prognosis of IPF.Methods The expressions of the proteins EGFR,STAT1 and ERK1/2 were assayed by an immunohistochemical method in 15 IPF specimens and 10 normal lung tissues.Results The expression rates of EGFR and STAT1 in the IPF group were 73.3% and 66.7%,respectively.The stained points of EGFR and STAT1 were positively correlated with the total lung capacity,diffusion capacity and PaO2 level（all P0.05）,but not correlated with the pathological characteristics or imaging features of high-resolution chest CT.EGFR and STAT1 were not significantly expressed in the normal lung tissues.ERK1/2 expression in the IPF group was not significantly increased compared with that in the normal lung tissues group（P0.05）.Conclusion The activation of the EGFR/STAT signal transduction pathway is closely related to the occurrence,progression and metastasis of IPF.Proteins such as EGFR and STAT1 can be used as valuable indicators for assessing the progression of IPF.

关 键 词：特发性肺纤维化 表皮生长因子受体(EGFR) ERK1 2 STAT1 肺功能 

分 类 号：R563[医药卫生—呼吸系统]