纳米银对小鼠流感治疗作用的研究  

Therapeutic effect of silver nanoparticles against influenza in mice

作　　者：李秀景[1] 尹俭俭[1] 郑丛龙[1] 

机构地区：[1]大连大学医学院病原生物学教研室,辽宁大连116622

出　　处：《大连医科大学学报》2013年第3期223-228,共6页Journal of Dalian Medical University

基　　金：国家自然科学基金项目(30870681)

摘　　要：目的探讨不同浓度的纳米银对小鼠流感的治疗作用。方法选择18～22 g健康昆明小鼠48只,随机分成6组:正常对照组、模型组、治疗阳性对照组、高剂量组(纳米银400μg/mL)、中剂量组(纳米银100μg/mL)和低剂量组(纳米银25μg/mL)。小鼠感染H1N1流感病毒A/PR/8/34(PR8 H1N1),4 h后鼻腔给予不同浓度纳米银,连续治疗3 d。记录各组小鼠每天的一般生理状况以及生存和死亡情况,观察14 d;通过比较各组小鼠的死亡率、生存天数、肺指数和肺组织病毒滴度,观察各组小鼠肺组织病理变化,测定各组小鼠肺组织的细菌感染情况,研究不同浓度纳米银对小鼠流感的疗效。结果高剂量组、中剂量组和低剂量组的死亡率均明显减低,其平均生存天数均明显延长,与模型组比较差异有显著性意义,P<0.01;高剂量组、中剂量组和低剂量组的肺指数均有所降低,且高剂量组和中剂量组与模型组比较差异有显著性意义,P<0.01;模型组小鼠肺组织的病毒滴度为1∶512,有的小鼠高达1∶1024,高剂量组、中剂量组和低剂量组的大部分小鼠的肺组织病毒滴度<1∶2。模型组小鼠肺组织的形态结构消失,肺泡壁增厚,组织间可见大量红细胞,肺泡腔见水肿液;高剂量组小鼠肺组织的病变明显减轻,肺组织形态结构较为完整,肺泡腔中无水肿液。模型组携菌量较多,高剂量组、中剂量组和低剂量组的携菌量减少,与模型组相比差异有显著性意义,P<0.01。结论纳米银对小鼠流感具有明显的治疗作用,其治疗效果与纳米银的剂量有关。Objective To discuss the therapeutical effect of different concentrations of silver nanoparticles against influenza in mice.Methods Forty-eight Kun Ming mice,which were healthy and weigh 18-22 g,were randomly divided into normal control group,model group,positive control group,high dose group（the concentration of silver nanoparticles is 400 μg/mL）,middle dose group（100 μg/mL） and low dose group（25 μg/mL）.Mice were infected with the influenza virus A/PR/8/34 H1N1（PR8 H1N1） and intranasal administration of silver nanoparticles at different concentrations after 4 hours,and treated continuously 3 days.Record the general physical condition,the condition of life and death count of each group every day for 14 days.To study the curative effect of silver nanoparticles at different concentrations against influenza in mice,the indexes that mortality rate,survival days,lung index,lung virus titer,lung histopathological examination and lung bacterial infections of each group were used.Results Compared with model group,the mortality of high,middle and low dose group reduced obviously,and monse survival days in these gvoup were significantly longer（P0.01）.The lung index of high,middle and low dose group reduced and the high and middle dose group reduced significantly（P0.01）.The lung virus titer of model group was 1∶512,and some mice were as high as 1∶1024;the with hist in the high,middle and low dose group was 1∶2.In model group,the morphological structure of lung tissue was disappeared,the alveolar walls were thickened,a large number of red blood cells wlhe found,and the edema fluid was found in alveolar cavity.In high dose group,the pathological changes of lung tissue were obviously alleviated,and it is no edema fluid in alveolar cavity.There were large numbers of bacteria in model group,the bacterial content in high,middle and low dose group reduced significantly（P 0.01）.Conclusion Two silver nanoparticles would hmte therapeutic effect on influenza in mice,and the curative effect was dose

关 键 词：纳米银 流感 小鼠 

分 类 号：R285.5[医药卫生—中药学]