2-KGA混菌发酵大小菌耦合关系研究及建模  

Modeling on interaction of different strains in 2-KGA mixed culture fermentation

作　　者：丰强强[1] 潘玉霖[1] 成宝琨[1] 孙君伟 袁景淇[1] 

机构地区：[1]上海交通大学自动化系,系统控制与信息处理教育部重点实验室,上海200240 [2]河北维尔康制药有限公司,河北石家庄050031

出　　处：《化工学报》2013年第7期2520-2525,共6页CIESC Journal

基　　金：博士学科点专项科研基金项目(20110073110018);国家自然科学基金项目(60974068)~~

摘　　要：研究了维生素C前体2-酮基-L-古龙酸(2-KGA)混菌发酵巨大芽孢杆菌(俗称大菌)与普通生酮古龙酸菌(俗称小菌)的耦合关系。经研究发现:(1)大菌通过群体感应调整自身生长行为;(2)大菌活菌代谢物及其自溶物质弥补小菌代谢缺陷促进小菌生长;(3)小菌可能通过释放溶菌酶促使大菌自溶;(4)大菌自溶释放一些蛋白酶类物质提高小菌山梨糖脱氢酶(SDH)活性从而加速产物形成。基于以上研究建立了2-KGA混菌发酵的动力学模型,并用实验数据证明了模型的有效性。The interaction between Bacillus megaterium （ also called big bacterium ） and Ketogulonicigenium vulgare （also called small bacterium） was studied for 2-keto-L gulonic acid （2 KGA） mixed culture fermentation. The following mechanisms were found. （1） Big bacterium might adjust its g b rowth behavior by quorum sensing. （2） Metabolites and autolysis substances of big bacterium were growth. （3） Small bacterium released lysozyme to promote big bacterium＇s autolysis. （4） Big bacterium increase the synthesis rate of 2-KGA. Based on such mechanisms, a kinetic model of 2-KGA mixed fermentation was established. Model validation was carried out with four sets of experimental data under different cultivation conditions. The results demonstrated that the proposed model was able to well describe the growth of two bacteria and 2-KGA production.

关 键 词：2-KGA 混菌发酵 群体感应 信号耦合 代谢耦合 动力学模型 

分 类 号：TP273[自动化与计算机技术—检测技术与自动化装置]