低剂量照射促进骨痂矿化的机制  

Effects of low-dose-X-irradiation on vascular endothelial growth factor and bone marrow mesenchy- real stem cells

作　　者：邓晔坤[1] 顾军[1] 周晓中[1] 宋祥胜 刘洪鹏[1] 余昶[1] 董启榕[1] 

机构地区：[1]苏州大学附属第二医院骨科,215004

出　　处：《中华实验外科杂志》2013年第7期1377-1379,共3页Chinese Journal of Experimental Surgery

基　　金：基金项目：国家自然科学基金资助项目（30872637、81171712）;“江苏省医学重点人才（2011-15）”资助项目;江苏省“十二五”临床医学重点学科资助项目;江苏省研究生科研创新资助项目（CXLX11_0087）

摘　　要：目的观察低剂量照射（LDI）对血管内皮生长因子（VEGF）和骨髓间充质干细胞（BMSCs）的影响，探讨其促进骨痂矿化的机制。方法LDI干预下，分别分析血清和骨痂内VEGF表达的变化，同时检测原代BMSC的成骨分化情况。结果实验组血清VEGF含量在术后第3周达（315．00±21．33）ng／L，明显高于对照组（175．05±18．17）ng／L（P〈0．05）。术后第2和第3周，实验组骨痂内VEGFmRNA的表达和新生血管数量明显高于对照组（P〈0．05）；10cGy的LDI可明显刺激BMSCs的增殖（1．697±0．048，照射后24h）（P〈0．05）。10cGy实验组矿化结节数目[（24．0±1．6）个]较对照组明显增多（P〈0．05），且骨保护素基因（OPG）、I型胶原（COL-1）、骨钙素基因（BGP）的表达也明显高于对照组（P〈0．05）。结论LDI通过上调VEGF的表达和促进BMSCs的动员，促进了骨痂的矿化。Objective To explore the mechanism of low-dose X-irradiation （LDI） promoting cal- lus mineralization through vascular endothelial growth factor （VEGF） and bone marrow mesenchymal stem cells （BMSCs）. Methods The expression of VEGF in serum and callus samples was quantified and the osteogenic differentiation of BMSCs was detected after exposure. Results MicroCT based angiography visu- ally revealed that the number of neovascularization was conspicuously increased since 2 weeks in the LDI group [ （315.00 ±21.33） ng/L] as compared with that in sham group [ （ 175.05 ~ 18. 17） ng/Ll （P 〈 0. 05）. The serum VEGF in the LDI group was higher than in control group （P 〈0. 05） at 3rd week. Elevat- ed mRNA expression of VEGF was observed, and reached the peak at 2nd and 3rd week respectively in the LDI group as compared with control group （ P 〈 0. 05 ）. The cell counting kit-8 （ CCK-8 ） assay showed that the proliferation of BMSCs was accelerated significantly in vitro in the 10 cGy irradiation group （ 1. 697 _± O. 048, 24 h after exposure） （ P 〈 0. 05 ）. Mineralized nodule number in the LDt group [ （24. 0 ± 1.6） ] was greater than in control group, especially in the 10 cGy group （P 〈0. 05）. RT-PCR showed that the expres- sion of Osteoprotegerin （ OPG）, collagenase-1 （ COL-1 ）, and osteocalcin （BGP） genes was increased in the LDI groups, especially in the 10 cGy group （P 〈 O. 05 ）. Conclusion The results indicate that LDI promotes callus mineralization through up-regulating VEGF and promoting the mobilization of BMSCs.

关 键 词：低剂量照射 血管内皮生长因子 骨髓间充质干细胞 

分 类 号：R6[医药卫生—外科学]