响应面法优化超声辅助提取太子参多糖工艺研究  

Optimization of Ultrasonic-assisted Extraction Conditions of Polysaccharides from Radix Pseudostellariae by Response Surface Methodology

作　　者：林志娟[1] 陈永[1] 尤丽彤[2] 孙凤祥[1] 李桂芝[1] 

机构地区：[1]潍坊医学院基础医学院 [2]潍坊医学院2008级药学专业,潍坊261053

出　　处：《天然产物研究与开发》2013年第6期846-850,共5页Natural Product Research and Development

基　　金：山东省自然科学基金(ZR2011HQ044);潍坊市科技发展计划(201101178);山东省高等学校科技计划项目(J12LKO2)

摘　　要：本文探讨超声波作用下太子参多糖提取的最佳工艺条件。在单因素试验基础上,采用响应面法对太子参多糖提取工艺参数进行优化研究。响应面试验表明提取温度、提取时间和水料比对响应值多糖提取得率均有显著影响,优化得到太子参多糖超声提取最佳工艺条件为:提取温度为74℃;提取时间为65 min;水料比为26 mL/g;超声波功率100 W。在此条件下太子参多糖的提取得率为2.48%,与模型预测值非常接近。To obtain the optimal conditions of ultrasonic-assisted extraction of polysaccharides from Radix PseudosteUari- ae, on the basis of the single factor test, the response surface methodology was applied to optimize the main extraction conditions. The results showed that extraction temperature, extraction time and liquid-solid ratio had significant effects on extraction yield of polysaccharides. The optimum conditions for the ultrasonic assisted extraction technique of polysaccharide from Radix Pseudostellariae were ： extraction temperature of 74℃, extraction time of 65 min, ultrasonic power of 100 W, ratio of liquid to solid of 26 mL/g. Under these optimized conditions, the extraction yield of polysaccharides was 2. 48%, exhibiting a good agreement with the predicted values.

关 键 词：太子参多糖 提取 超声波 响应面法 

分 类 号：R284.2[医药卫生—中药学]