兔心肌梗死再灌注和血管损伤双模型的建立与评价  

Establishment and evaluation of rabbit myocardial infarction reperfusion with vascular injury model

作　　者：王正龙[1] 刘志江[1] 赵然尊[1] 郭艳[1] 石蓓[1] 

机构地区：[1]遵义医学院附属医院心血管内科,贵州遵义563099

出　　处：《遵义医学院学报》2013年第3期193-197,共5页Journal of Zunyi Medical University

基　　金：国家自然科学基金资助项目(NO:81060014)

摘　　要：目的制作兔心肌梗死再灌注和血管损伤双模型,为同步研究心肌梗死后心肌重塑和血管重塑提供可行的动物模型。方法选用新西兰大白兔45只,随机分成2组:对照组(n=5)和模型组(n=40)。模型组用高脂饲料饲养建立动脉粥样硬化模型,然后制备心肌梗死再灌注和颈动脉球囊损伤双模型。采用心电图、心肌标志物和组织病理学评价心肌梗死再灌注模型;采用组织病理学评价颈动脉球囊损伤模型。结果心肌标志物升高并随时间变化呈动态演变,而且心肌随时间变化呈凝固性坏死到纤维疤痕改变;右颈总动脉球囊损伤后内膜增生。结论心肌梗死再灌注和血管损伤双模型的建立具有可行性。Objective To establish rabbit myocardial infarction reperfusion with vascular injury model. Methods Forty -five New Zealand white rabbits were randomly divided into the control group （n = 5 ） and model group （ n = 40） fed with high - fat diets to establish atherosclerosis model followed by the my- ocardial infarction reperfusion - induced injury by thoracotomy and ligating left ventricular branch of cor- onary artery and the subsequent carotid artery balloon injury. ECG, cardiac markers and histopathologi- cal examinations were performed to evaluate the model establishment. Results Compared with the control group, the cardiac markers were time - dependently increased with dynamic development and the time - dependent change from necrosis to fibrous scar was presented and carotid artery showed intimal hyperpla- sia in the model group. Conclusion It is feasible to eatablish myocardial infarction reperfusion with vas- cular injury model.

关 键 词：心肌梗死再灌注 血管损伤 模型 兔 

分 类 号：R541[医药卫生—心血管疾病]