质量权重系数整合法表征整体银杏黄酮组分在水中及不同pH磷酸盐缓冲液中的平衡溶解度  

Equilibrium solubility of ginkgo flavonoid components in water and PBS of different pHs with mass fraction weight coefficient method

作　　者：刘丹[1,2] 贾晓斌[1,2] 萧伟[3] 

机构地区：[1]江苏省中医药研究院国家中医药管理局中药释药系统重点研究室,江苏南京210028 [2]江苏大学药学院,江苏镇江212013 [3]江苏康缘药业股份有限公司中药制药过程新技术国家重点实验室,江苏连云港222001

出　　处：《中国中药杂志》2013年第12期1871-1875,共5页China Journal of Chinese Materia Medica

基　　金：国家"十一五"科技支撑计划项目(2008BAI51B03);中药制剂过程新技术国家重点研究项目(SKL2010Z0303);江苏省中医药局中医药科技技术研究专项(LZ09061)

摘　　要：目的:整合表征银杏黄酮组分整体在水中及不同pH的磷酸盐缓冲液(PBS)中平衡溶解度。方法:采用HPLC同时测定银杏叶提取物中槲皮素、山柰酚、异鼠李素3种黄酮类成分的含量和3类成分在水和不同pH缓冲溶液中的平衡溶解度,进一步通过质量分数权重系数整合法,整合表征银杏黄酮组分的平衡溶解度和油水分配系数。结果:银杏黄酮组分水溶性良好,在pH 7.8时平衡溶解度达到最大,即408.29 mg.L-1。可以初步预测银杏黄酮组分有较好的应用价值,为其组分的制剂成型提供一定的指导依据。结论:比较直接加和法和质量分数权重系数整合法表征银杏黄酮组分性质值,发现质量分数权重系数整合表征中药组分性质更为科学合理,通过该尝试性试验可以为中药组分的性质表征提供一定的研究思路。Objective： To descript the equilibrium solubility of ginkgo flavonoid components in water and PBS of different pHs. Method： The HPLC method was adopted to determine the concentration of quercetin, kaempferol and isorhamnetin in ginkgo bi- loba extracts, and the equilibrium solubility of the three components in water and PBS of different pHs. Furthermore, the mass fraction weight coefficient method was adopted to express the integrated equilibrium solubility and oil-water distribution coefficient of ginkgo fla- vonoid components. Result： Ginkgo flavonoid components were well dissoluble in water, with the maximum equilibrium solubility of 408.29 mg. L-1 at pH 7. 8. Therefore, it could be preliminarily predicted that ginkgo flavonoid components had higher application value, and could provide guiding basis for further development of preparations. Conclusion： By comparing the results of the direct ad- dition method and the mass fraction weight coefficient method, we found that the mass fraction weight coefficient method was more sci- entific and reasonable. The tentative study could provide ideas to property characterization of traditional Chinese medicine components.

关 键 词：质量分数权重系数整合法 中药组分 整体平衡溶解度 整体表观油水分配系数 

分 类 号：R284[医药卫生—中药学]