细胞膜色谱法在中药研究中的应用进展  

Cell membrane chromatography and its application progress in studies of traditional Chinese medicines

作　　者：张聪聪[1,2] 陈刚[3] 刘家兰[1] 李烨[1] 徐晓玉[1] 

机构地区：[1]西南大学药学院,国家中医药管理局中药药理学重点学科建设单位,重庆400716 [2]郑州市骨科医院药剂科,河南郑州450052 [3]重庆工商大学药物研究中心,重庆400067

出　　处：《中国中药杂志》2013年第12期1881-1886,共6页China Journal of Chinese Materia Medica

基　　金：教育部教师科研基金项目(XDJK2009C084,XDJK2010C059);重庆市自然科学基金项目(CSCT2011jjA0324)

摘　　要：细胞膜色谱法(cell membrane chromatography,CMC)是由贺浪冲教授在1996年创建的一种新型的具有生物活性的亲和色谱技术。该技术将高效液相色谱、细胞生物学与受体药理学结合起来,通过将活性细胞膜固定在特定载体表面,制备成细胞膜固定相,用液相色谱的方法研究药物与固定相上细胞膜及膜受体的相互作用,利用药物与膜受体间存在的特异亲和力,将药物在体内的作用过程在色谱柱内进行动态模拟。该文重点针对CMC的原理、特性及在中药等方面的典型应用研究进行概述,为进一步深入研究和开发利用CMC提供理论依据和实践指导。As a novel bioaffinity chromatography technique, cell membrane chromatography （CMC） was first established by Professor He in 1996, with which combined high performance liquid chromatography, cytobiology, and receptor pharmacology. The cell membrane stationary phase （CMSP） consists of porous silica coated with active cell membranes. By immersing silica into a suspension of cell membranes, the whole surface of silica was covered by the cell membranes. In CMC, the interaction of drugs or compounds with the immobilized cell membrane or its receptors is investigated using liquid chromatography. In general, with the aim to provide scientif- ic foundation for further development and application, this paper mainly focuses on the characteristics of the cell membrane stationary phase （CMSP）, the CMC analytical system, and its applications in traditional Chinese medicines（TCMs） about CMC. With the devel- opment of CMC, the breakthrough progress of it in studying active components of TCMs field is expectant.

关 键 词：细胞膜色谱法 中药 原理 特性 应用 

分 类 号：R28[医药卫生—中药学]