薏苡仁油对UUO大鼠肾间质纤维化的影响  

Effect of Coicis Semen oil on renal interstitial fibrosis in rats with unilaterai urethral obstruction

作　　者：胡颖[1] 林斐莉[1] 邹庆玲[1] 牟利军[1] 

机构地区：[1]浙江大学医学院附属第二医院肾内科,浙江杭州310006

出　　处：《中国中药杂志》2013年第12期1982-1986,共5页China Journal of Chinese Materia Medica

基　　金：浙江省中医药管理局项目(2009CA066)

摘　　要：目的:观察薏苡仁油对单侧输尿管梗阻大鼠肾间质纤维化的影响。方法:雄性SD大鼠54只随机分为假手术组、单侧输尿管结扎模型组和薏苡仁油治疗组(15 mL.kg-1.d-1),术后第3,7,14天分批处死大鼠。用HE和Masson染色光镜下观察肾间质的病理改变,用免疫组织化学方法检测肾组织α-平滑肌肌动蛋白(α-SMA)和转化生长因子β1(TGF-β1)表达,用Western blot法检测肾组织Smad2,Smad7蛋白的表达。结果:薏苡仁油能够改善梗阻侧肾小管间质纤维化评分,显著减少肾组织α-SMA和TGF-β1的表达,治疗组肾组织磷酸化Smad2蛋白表达显著低于模型组,而Smad7蛋白水平则明显高于模型组。结论:薏苡仁油具有抗肾间质纤维化的作用,其机制与降低肾组织中促纤维化细胞因子TGF-β1含量,抑制Smad2的磷酸化和恢复拮抗蛋白Smad7的表达有关。Objective： To study the effect and mechanism of Coicis Semen oil （Kanglaite injection, KLT） on renal interstitial fibrosis induced by unilateral ureteral obstruction （UUO）. Method： Fifty-four male SD rats were randomly divided into 3 groups, 6 in each group, the sham operated group, the model group, and the KLT group. Renal interstitial fibrosis model was established in rats by UUO. After administration of KLT （15 mL. kg-1 . d-1） for 3, 7 and 14 days, the dynamic histological changes of renal interstitial tissues were observed and renal damage including tubular impairment and interstitial fibrosis were quantified on HE and Masson stained tissue sections. The expression of a-smooth muscle actin（α-SMA）and transforming growth factor-betal （TGF-β1） were measured by immunohistochemistry staining sections. The protein expression of p-Smad2 and Smad7 were detected by Western blot respectively, lee- suit： The degree of tubular damage in KLT group was much lower than that in UUO group （P 〈 0. 05 ）. The expression of a-SMA and TGF-fll was decreased in both UUO group and KLT group, while it was significantly lower in KLT group at every time point. The pro- tein expression of p-Smad2 was obviously decreased while the protein expressions of Smad7 was obviously increased in KLT group, compared with the UUO group （P 〈 0. 05 ） . Conclusion： Coicis Semen oil could attenuate the tubulo-interstitial fibrosis, probable by intervening the TGF-β/Smads signal transduction pathway of UUO rats.

关 键 词：薏苡仁油 康莱特注射液 肾间质纤维化 α-平滑肌肌动蛋白 转化生长因子β1 SMAD2 SMAD7 

分 类 号：R285.5[医药卫生—中药学]