应用弱阳离子交换蛋白质芯片捕获的健康成人血清蛋白质/多肽谱分析  

Analysis of serum protein / peptides spectrum of healthy adult captured with weak cation exchange protein chip

作　　者：张为民[1,2] 谢亚琴[1,2] 龚文波[1,2] 

机构地区：[1]广东省深圳市人民医院 [2]暨南大学第二临床学院检验医学部,518020

出　　处：《实用医学杂志》2013年第13期2203-2205,共3页The Journal of Practical Medicine

基　　金：深圳市科技局立项项目(编号:200903003)

摘　　要：目的:分析健康成年人血清蛋白质/多肽谱特点,为人类血清蛋白质组研究提供基础性资料。方法:应用弱阳离子交换蛋白质芯片(WCX2)磁珠处理30例健康成年人血清,通过表面增强激光解吸-电离飞行时间质谱(SELDI-TOF-MS)技术检测血清蛋白质/多肽谱,描述性分析其种类、分子质量范围、丰度(用相对强度计量)、个体性差异等。结果:共检测到947种蛋白质/多肽,不同个体其种类数量在34～108不等,相对分子质量范围为1059～10265,表达丰度在95～35419之间;分别有570、154、71、36、32、26、18、16、14、5、2和3种相应血清蛋白质/多肽在1、2、3、4、5、6、7、8、9、10、11、14例个体血清中存在。结论:WCX2磁珠仅能捕获到数量较少的低丰度、低分子量人血清蛋白/多肽,在受检人群中这些蛋白/多肽呈现高度的个体差异性。Objective To analyze the serum protein/peptides spectrum characteristics of healthy adult and to provide basic information for human serum proteomic study. Methods Serum protein/peptides of 30 healthy adult linked to weak cation exchange protein chip （WCX2 bead） were detected by surface-enhanced laser desorption/ ionization time-of-flight mass spectrometry （SELDI-TOF-MS）. And the experimental data, inclucling the kinds, range of relative molecular mass, abundance measured in relative strength and individual variety, were analyzed descriptively. Results 947 kinds of protein/peptides, whose relative molecular masses range from 1 059 to 10 265 were found out and there were 34 - 108 kinds of protein/peptides in various individuals. The abundance of these serum protein/peptides were in the range of 95 to 35 419. As to individual variety, 570, 154, 71, 36, 32, 26, 18, 16, 14, 5, 2 and 3 kinds of serum protein/peptides existed in 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 14 subjects, respectively. Conclusions WCX2 bead can only capture these human serum protein/peptides of which the quantity, the abundance and the relative molecular mass is less, smaller and lower than that of being. Furthermore, they present significant individual variety among subjects.

关 键 词：血蛋白质类 弱阳离子交换蛋白质芯片 表面增强激光解吸-电离飞行时间质谱 

分 类 号：R446.1[医药卫生—诊断学]