Screening and identification of an antagonistic bacterium strain LHB02 and the optimization of its fermentation conditions  

一株生防细菌LHB02的筛选、鉴定及其发酵条件优化(英文)

作　　者：徐长安[1] 唐旭[1] 何建林[1] 林凌[1] 刘源森[1] 方卫东[2] 

机构地区：[1]国家海洋局第三海洋研究所,福建厦门361005 [2]集美大学水产学院,福建厦门361015

出　　处：《Marine Science Bulletin》2013年第1期39-50,共12页海洋通报（英文版）

基　　金：supported by the Scientific Research Foundation of Xiamen Marine Research and Development Institute (No. K10102 (1))

摘　　要：A strain LHB02 with strong antibacterial activity against some aquatic pathogens was screened from the coastal marine sediment in Fujian province, China. LHB02 was identified as Bacillus subtilis, based on its 16S rRNA sequence, together with the morphological, physiological and biochemical characteristics. The antagonistic activity of strain LHB02 and its optimal fermentation conditions were also investigated. The results showed that LHB02 had strong antagonistic activity against 3 species of vibrios： Vibrio harveyi,Vibrio alginolyticus and Vibrio anguillarum, and the optimum fermentation conditions for the strain LHB02 were as follows： KB culture medium （peptone 20 g, glycerol 10 mL, K2HPO4 1.5 g, MgSO 4 .7H2O 1.5 g, H2O 1 000 mL）; temperature, 28 ℃; pH ,7.0; culture time, 36 h; and inoculation amount, 1.5% （v/v）.从中国福建沿海某一污染滩涂的沉积物中筛选到一株具有较强拮抗水产病原菌的生防细菌LHB02。采用通用引物,对LHB02的16SrRNA片段进行扩增,获得1511bp的DNA系列,基于该DNA系列,结合细菌形态学、生理生化特征,鉴定LHB02为一株枯草芽孢杆菌。对LHB02进行了抗菌试验、抗菌谱检测和发酵条件优化研究,结果表明,LHB02对哈维氏弧菌、溶藻弧菌、鳗弧菌等有很强的拮抗作用,其优化后的发酵条件为:KB培养基(蛋白胨20g,丙三醇10mL,K2HPO41.5g,MgSO4.7H2O1.5g,H2O1000mL);温度28°C,pH值7.0,培养时间36h,接种量1.5%。

关 键 词：SCREENING IDENTIFICATION bacillus subtilis antagonistic activity OPTIMIZATION 

分 类 号：S917.1[农业科学—水产科学]