外源性磷酸肌酸对游泳力竭小鼠大脑中谷氨酸和钙-ATP酶活力的影响  

Effects of exogenous creatine phosphate on glutamic acid and Ca^(2+)-ATPase activity in brain of mice after exhaustive exercise

作　　者：马集 卢畅[1] 姜茜[1] 殷林波[1] 刘彦娜[2] 刘克敏[2] 

机构地区：[1]大连医科大学医学检验系,辽宁大连116044 [2]大连医科大学基础医学院运动医学教研室,辽宁大连116044

出　　处：《中国康复》2013年第3期167-170,共4页Chinese Journal of Rehabilitation

基　　金：大连医科大学大学生科技创新活动基金资助项目(2013022)

摘　　要：目的:研究外源性磷酸肌酸(PCr)对游泳力竭小鼠大脑中谷氨酸(Glu)和钙-ATP酶(Ca2+-ATPase)活力的影响,以进一步揭示PCr的抗疲劳机制。方法:将44只6周龄小鼠分为力竭对照组12只(A组)、力竭给药组12只(B组)、游泳8min对照组10只(C组)、游泳8min给药组10只(D组),采取小鼠负重游泳的力竭运动模型,每只小鼠负重量为自身体质量的6%。于游泳前30min,B、D组小鼠经腹腔注射磷酸肌酸钠溶液1000mg/kg;A、C组小鼠注射同等比例生理盐水作为安慰剂。记录力竭组小鼠的力竭游泳时间,采用化学比色法检测4组小鼠大脑中Glu含量和Ca2+-ATPase的活性。结果:经检测,B组小鼠的力竭游泳时间明显长于A组(P<0.05)。小鼠大脑Glu含量检测显示,B组明显低于A组,D组明显低于C组(P<0.05);Ca2+-ATPase活力检测显示,B组明显高于A组,D组明显高于C组(P<0.05)。结论:外源性PCr的抗疲劳机制与增强Ca2+-ATPase活性和间接降低大脑中的Glu含量有关。Objective：To observe the effects of exogenous ereatine phosphate on glutamic acid level and Ca2＋-ATP- ase activity in brain of mice after exhaustive exercise and to further reveal the mechanism of exogenous creatine phos- phate in allaying tiredness. Methods：All 36 mice, 6-week-age,were divided into 4 groups：exhaustive swimming con- trol group （group A）; exhaustive swimming with medication group （group B）; 8-min swimming control group （group C）;and 8-min swimming with medication group （group D）. The method of mice weight-loading swimming was used to sets up the model of exhaustive exercise,and each mouse loaded weight with 6 % of the mass of itself. Thirty min before the experiment, mice in groups B and D were given the intraperitoneal injection with creatine phos- phate sodium by the standard of 1000 mg/kg,and the mice in groups A and C were given the same proportionate normal saline as placebo. The exhaustive swimming time was recorded,and glutamic level and Ca2＋-ATpase activity were measured by using biochemical kits. Results：After testing,the exhaustion time in group B was longer than that in group A （P〈0.05）. The Glu contents in groups B and D were significantly lower than in groups A and C （P〈 0.05）. Ca2＋-ATPase activity in groups B and group D was significantly higher than that in groups A and C （P（ 0.05）. Conclusion：The mechanism of exogenous creatine phosphate in allaying tiredness may be closely related with increased Ca2＋-ATPase activity and reduced glutamic level.

关 键 词：运动性疲劳 磷酸肌酸 谷氨酸 钙-ATP酶 

分 类 号：R49[医药卫生—康复医学] R87[医药卫生—临床医学]