茶叶中掺杂小麦DNA的提取及检测方法的比较  

Comparison of Extraction and Detection of DNA of Wheat in Tea

作　　者：张吉红[1] 潘登[2] 余澍琼[1] 陈建国[1] 

机构地区：[1]宁波出入境检验检疫局,浙江宁波315012 [2]浙江万里学院生物环境学院,浙江宁波315100

出　　处：《贵州农业科学》2013年第6期148-150,154,共4页Guizhou Agricultural Sciences

基　　金：宁波检验检疫局科研项目"茶叶掺杂使假的现代鉴定技术研究"(甬K04-2011)

摘　　要：为探索茶叶中掺杂小麦的快速检测方法,对3种不同的核酸提取方法(标准CTAB提取法、改良CTAB提取法和试剂盒提取法)进行分析比较,根据小麦内源GAG56D基因比较了茶叶中掺杂小麦成分的普通PCR和实时荧光PCR检测方法。结果表明:最适合掺杂小麦DNA的方法为标准的CTAB提取法。扩增结果显示,针对理论DNA浓度的检测灵敏度,实时荧光PCR的灵敏度能达到0.1%,普通PCR能达到1%,实时荧光PCR是普通PCR的10倍。To explore a rapid detection method of wheat in tea,three nucleic acid extraction methods（standard CTAB extraction method,modified CTAB extraction method and kit assay） were analyzed and compared.According to the endogenous gene GAG56D of wheat,the PCR and real-time PCR methods were used to detect wheat in tea.The results showed that standard CTAB extraction method was the most suitable by comparing with the other two different methods.The DNA sensitive results showed that sensitive of real-time PCR reached 0.1%,while PCR reached 1%.The sensitive of the former was 10 times of the later.

关 键 词：茶叶 小麦 DNA提取 普通PCR 实时荧光PCR 检测方法 

分 类 号：S338[农业科学—作物遗传育种] Q943.2[农业科学—农艺学]