沙葱多糖对绵羊外周血淋巴细胞的调节作用研究  

Study on the regulating effect of Allium mongolicum regel polysaccharides on peripheral blood lymphocytes of sheep

作　　者：包美艳[1] 敖长金[1] 赵飞艳[1] 萨茹丽[1] 

机构地区：[1]内蒙古农业大学动物科学学院,内蒙古呼和浩特010018

出　　处：《饲料工业》2013年第12期9-12,共4页Feed Industry

基　　金：国家自然科学基金[30760173]

摘　　要：试验研究沙葱多糖（Allium mongolicum regel polysaccharides）对绵羊外周血淋巴细胞增殖及一氧化氮（NO）、诱导型一氧化氮合酶（iNOS）的影响。试验设置不同浓度（0—250μg／ml）的沙葱多糖和不同的作用时间（24、48、72h），以MTT比色法检测淋巴细胞增殖；以培养24h的绵羊外周血淋巴细胞为研究对象，以微板法、硝酸还原酶法分别测定NO及iNOS。结果表明，沙葱多糖浓度为50～100μg／ml时抑制淋巴细胞的增殖，当浓度为150～250μg／ml时促进淋巴细胞的增殖，且作用时间为24h时增殖效果最强。较低浓度的沙葱多糖（50～100μg／m1）抑制淋巴细胞分泌NO，随着浓度的加大，NO分泌量增加。沙葱多糖对淋巴细胞内iNOS活性没有显著影响（P〉0．05）。因此，沙葱多糖能够影响淋巴细胞的增殖，释放NO及iNOS，并且呈现量效和时效关系，说明沙葱多糖对绵羊外周血淋巴细胞具有免疫调节作用。The objective of this study was to determine the effects of allium mongolicum regel polysaccharides on lymphocyte proliferation, nitric oxide（NO） content and inducible nitric oxide synthase（iNOS） in peripheral blood of sheep. With MTT colorimetric assay to measure lympho- cyte proliferation in different functioning time and different allium mongolicum regel polysaccha- rides concentration. With microplate and nitratase method to measure NO and iNOS in peripher- al lymphocytes cells of sheep which were cultured for 24 hours. The allium mongolicum regel polysaccharides inhibited lymphocyte proliferation when the concentration was 50-100 μg/ml, but it improved lymphocyte proliferation when the concentration was 150-250 μg/ml and the highest lymphocytes proliferation at 24 h. Allium mongolicum regel polysaccharides with low con- centrations（50-100 μg/ml） inhibited lymphocyte secretion of NO, and NO secretion increased with the increase of concentration.There had no significant effect on lymphocyte secretion of iN- OS by allium mongolicum regel polysaccharides（P〉0.05）. Allium mongolicum regel polysaccha- rides had effect on lymphocytes proliferation, and it also can release NO and iNOS, these had re- lationship with dose and effective functions time. It showed that allium mongolicum regel poly- saccharides had immune regulation function to lymphocyte in peripheral blood of sheep.

关 键 词：沙葱多糖 绵羊 外周血淋巴细胞 增殖 一氧化氮 诱导型一氧化氮合酶 

分 类 号：S816.7[农业科学—饲料科学]