大豆结荚习性、荚色和种皮色相关野生片段分析  

Identification of Wild Segments Associated with Stem Termination,Pod Color,and Seed Coat Color in Soybean

作　　者：王吴彬[1] 何庆元[1] 杨红燕[1] 向仕华[1] 邢光南[1] 赵团结[1] 盖钧镒[1] 

机构地区：[1]南京农业大学大豆研究所/国家大豆改良中心/农业部大豆生物学与遗传育种重点实验室(综合)/作物遗传与种质创新国家重点实验室,江苏南京210095

出　　处：《作物学报》2013年第7期1155-1163,共9页Acta Agronomica Sinica

基　　金：国家重点基础研究发展计划(973计划)项目(2009CB1184;2011CB1093);国家自然科学基金项目(31071442;30900902);国家公益性行业(农业)科研专项经费项目(200803060);江苏省优势学科建设工程专项和国家重点实验室自主课题;国家现代农业产业技术体系建设专项(CARS-04);农业部大豆生物学与遗传育种创新团队项目资助

摘　　要：结荚习性、荚色和种皮色是大豆的重要形态性状,与进化密切相关。利用由151个家系组成的野生大豆(Glycine soja Sieb et Zucc.)染色体片段代换系(CSSL)群体(SojaCSSLP1),通过不同表型CSSL组间比对,分别检测到与结荚习性、荚色和种皮色相关的1、3和2个野生片段(基因)。其中,5个野生片段(基因)分别与前人在栽培豆中检测到的无限结荚Dt1、荚色L1、荚色L2、绿种皮G和黑种皮i基因相对应,说明野生大豆与栽培大豆间、栽培大豆与栽培大豆间在这些片段上均存在等位变异分化,是与大豆进化相关的基因/片段。另一个与荚色相关的Satt273野生片段能使大豆表现黑荚,可能是本研究的新发现,但还需进一步验证。Stem termination （ST）, pod color （PC） and seed coat color （SCC） are important morphological traits, which are related to evolution in soybean. By using a wild soybean （Glycine soja Sieb et Zucc.） chromosome segment substitution line （CSSL） population, designated as SojaCSSLP 1 composed of 151 lines, one ST, three PC and two SCC wild segments/alleles were detected based on the comparison of different CSSL groups with a same phenotype on the respective trait. Among them, five wild al- leles/segments identified in this study were corresponding to Dtl, L2, L1, G and i, respectively, which indicated that there existed allele differentiation happened between wild and cultivated soybean as well as between cultivated soybeans on these loci/segments and that the genes/segments involved with domestication and evolution. The wild segment of Satt273 for PC might be a novel gene/segment, which needed further verification. The identification of the genes/segments provide basic materials for cloning the wild alleles, studying the wild allele function and recombination of the wild alleles/segments.

关 键 词：野生大豆 染色体片段代换系 荚色 结荚习性 种皮色 

分 类 号：S565.1[农业科学—作物学]