CD133功能化肝素／胶原蛋白多层膜改性小口径膨胀体聚四氟乙烯人造血管  

Surface Modified Small-Caliber ePTFE Grafts via CD133 Antibody Functioned Heparin/Collagen Multilayer

作　　者：张鹏[1] 龚飞荣[1] 陆树洋[2] 孙晓宁[2] 程树军[1] 

机构地区：[1]华东理工大学材料科学与工程学院,超细材料制备与应用教育部重点实验室,上海200237 [2]复旦大学附属中山医院,上海200232

出　　处：《华东理工大学学报（自然科学版）》2013年第3期257-261,274,共6页Journal of East China University of Science and Technology

基　　金：国家自然科学基金(81000105)

摘　　要：小口径膨胀体聚四氟乙烯(ePTFE)人造血管是血管移植手术中最常见的替代品之一,然而术后血栓的形成和血管内腔的再狭窄是导致手术失败的重要因素。采用静电层层自组装的方法构建CD133功能化肝素/胶原蛋白(HEP/COL)多层膜可以抑制血栓的形成,并且为血管的内皮化提供可能;通过扫描电镜、红外谱图跟踪HEP/COL多层膜的组装过程,利用激光共聚焦显微镜来证明抗体的接枝。结果发现,CD133功能化的HEP/COL多层膜成功地覆盖到了ePTFE人造血管表面;接触角从127°减小到106°,表明血管的浸润性得到提高;血小板吸附的实验结果表明,改性后的人造血管表面血小板吸附数量明显减少,具有良好的抗凝血性。Small caliber vascular grafts of expanded polytetrafluoroethylene （ePTFE） is one of the most common substitutes in vascular implantation surgery. However, late thrombosis and in vascular restenosis constitute the crucial factors that lead to long term implantation failure. Thrombosis inhibition can be achieved by buliding up CD133 functionalized heparin collagen （HEP/COL） multilayer, which also provides possibilities of the vascular re-endothelialization. The HEP/COL multilayer is constructed via layer-by-layer assembly technique and the assembly process is monitored by Fourier transform infrared spectrum （FT-IR）, scanning electron microscrope（SEM）. Confocal laser scanning microscropy is used for characterizing the successful immobilization of CD133 antibody. Finally, the CD133 antibody funetionalized HEP/COL multilayer was successfully coated on the ePTFE vascular surface. The result of contact angle experiment reveals great improvement in hydrophilicity, while water contact angle decreasing from 127° to 106°. The in vitro platelet adhension rate decreases dramatically after modification of the ePTFE vascular surface, indicating improved anticoagulant property

关 键 词：肝素 胶原蛋白 层层自组装 抗凝血性 

分 类 号：R318[医药卫生—生物医学工程]