蝴蝶兰染色体制片技术流程之探索  

Exploration on the Preparation Procedures of Using Enzyme Shed Cell Wall on Chromosome of Phalaenopsis

作　　者：庄东红[1,2] 曲莹[2] 杨培奎[1] 

机构地区：[1]韩山师范学院生物系,广东潮州521041 [2]汕头大学生物系,广东汕头515063

出　　处：《韩山师范学院学报》2013年第3期41-45,共5页Journal of Hanshan Normal University

基　　金：广东省自然科学基金资助项目(项目编号:2003C2011046);汕头市科技计划资助项目(项目编号:200543)

摘　　要：应用酶解去壁法制备蝴蝶兰染色体标本,对供试材料的选取、预处理方法、酶解时间和温度加以探索.实验结果显示,换盆后14～20 d的盆苗嫩根根尖分裂活性最高、中期分裂相较多.剪取长1 cm左右的根尖用0.002M的8-羟基喹啉在18℃预处理4 h,卡诺固定液固定2～24 h,再用4%纤维素酶和1%果胶酶的混合酶液在25℃下酶解1.5 h,能获得高质量的制片,染色体在载玻片上分散良好、形态清晰.By using shedding cell wall with enzyme to make the picture of karyotype of Phalaenopsis, We explored on the activity of plant materials, pretreatment, time and temperature of incubated in enzyme. The result indicated that the young root tips of Phalaenopsis after changing pot for 14-20 d are the best materials for experiment. The best preparation procedures are as follows： lcm long root tips were immersed in 0.002 M 8 - hydroxyquinolin for 4h at 18 %, fixed in Carnoy＇ s for 4-20 h at 4℃, incubated in a mixture of 4% cellulose and 1% pectolyase for 1.5 h at 25℃. The result showed that there were lots of scattering cell on the pictures. It was optimum for chromosome preparation because it allowed morphological characteristics to become visible.

关 键 词：蝴蝶兰 染色体 酶解去壁法 

分 类 号：Q343[生物学—遗传学]