推拿对坐骨神经损伤大鼠神经生长因子及其受体p75NTR的影响  

Investigation of tuina therapy on NGF and p75NTR of sciatic nerve injury model rats

作　　者：梅旭晖[1] 纪倩[1] 姚斌彬[1] 吴剑聪[1] 鲁梦倩[1] 于天源[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029

出　　处：《中华中医药杂志》2013年第7期1994-1997,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.30873311)~~

摘　　要：目的:观察推拿对坐骨神经损伤大鼠行为学、神经生长因子(NGF)及其受体p75NTR的影响,探讨推拿治疗坐骨神经损伤的生物学机制。方法:采用神经夹持损伤的方式建立大鼠坐骨神经损伤模型,观察各组大鼠的行为学变化,脊髓内NGF及p75NTR表达情况。结果:模型组和模型对照组大鼠行为学检测表明坐骨神经损伤后大鼠的运动及感觉功能明显降低(P<0.05),推拿治疗后斜板实验及热痛阈实验评分明显升高(P<0.05),与正常组无显著性差异;模型组、模型对照组及推拿组NGF及p75NTR免疫组化表达与正常组相比均有显著性差异(P<0.05),其中模型组及模型对照组NGF略升高(P<0.05),p75NTR显著性升高(P<0.05),推拿组NGF显著性升高(P<0.05),p75NTR显著性下降(P<0.05)。结论:推拿治疗可以通过提高机体NGF表达,降低NGF的低亲和力受体p75NTR释放,从而抑制细胞凋亡机制,最终改善坐骨神经损伤大鼠的运动及感觉功能。Objective： Observe the influence of tuina therapy to the NGF and p75NTR of sciatic nerve injury model rats. Probe the biological mechanisms of tuina therapy in sciatic nerve injury. Methods： Use the nerve clamping method to make the sciatic nerve injury model. Observe the oblique board test and thermal withdraw latency test as behavioral evidences. Observe NGF and p75NTR protein by using immunohistochemistry. Results： The oblique board test scores of the model group and sham- tuina group are significantly lower than blank control group （P〈0.05）, while tuina group score is much higher （P〈0.05）. The thermal withdraw latency test scores of the model group and sham-tuina group are significantly higher than blank control group （P〈0.05）, while tuina group score is much lower （P〈0.05）. The immunohistochemistry shows that the amounts of the NGF and p75NTR proteins are all significantly different in model control group, sham-tuina group and tuina group. NGF is significantly higher in tuina group, and p75NTR is lower in tuina group （P〈0.05）. Conclusion： Tuina therapy treats the sciatic nerve injury by increasing NGF, reducing p75NTR. As a result, the apoptosis mechanism of nerve cell is controlled by tuina therapy, so the feeling function and motor function of the rats are improved.

关 键 词：推拿 坐骨神经损伤 神经生长因子 P75神经营养因子受体 

分 类 号：R274[医药卫生—中医骨伤科学]