温郁金二萜类化合物C抑制由脂多糖诱导胃癌细胞炎症因子的释放及机制研究  

Inhibition and possible mechanism of curcuma wenyujin diterpenoid compound C on lipopolysaccharide-induced release of inflammatory factors in gastric cancer cell

作　　者：金海峰[1] 吕宾[1] 赵敏[1] 盛桂琴[1] 

机构地区：[1]浙江中医药大学附属第一医院,杭州310006

出　　处：《中华中医药杂志》2013年第7期2012-2015,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省自然科学基金项目资助(No.Y2110959)~~

摘　　要：目的:研究温郁金醚提物中新二萜类化合物C对由脂多糖(LPS)诱导胃上皮细胞炎症因子释放的影响及作用机制。方法:不同浓度的温郁金二萜类化合物C在不同的时间,体外作用于人胃癌SGC-7901细胞,用MTT法检测其对SGC-7901细胞的生长抑制率;用ELISA检测炎症因子IL-1β、IL-2的分泌;RT-PCR检测两种炎症因子mRNA转录情况;Western Blot方法检测p38、JNK、ERK蛋白量的表达变化。结果:温郁金二萜类化合物C在10μg/mL浓度以内、LPS在10ng/mL以内对SGC-7901增殖无影响;二萜类化合物C能显著性抑制由LPS诱导的炎症因子IL-1β释放(P<0.01),促进抑炎因子IL-2的释放(P<0.01);RT-PCR检测的结果与之相同;温郁金二萜类化合物C抑制MAPK通路中P38、JNK、ERK蛋白的表达。结论:温郁金二萜类化合物C能抑制由脂多糖诱导胃癌细胞炎症因子的释放,其作用机制可能与其抑制细胞内MAPK通路有关。Objective： To study the effect and possible mechanism of curcuma wenyujin diterpenoid compound C on lipopolysaccharide-induced （LPS-induced） release of inflammatory factors in gastric cancer cells. Methods： Human gastric cancer SGC-7901 cells were affected by curcuma wenyujin diterpenoid compound C with different concentrations in vitro at different times. The growth inhibition ratio of human gastric cancer SGC-7901 cells was measured by MTT assay, secretion of inflammatory factors IL-1β and IL-2 was detected by ELISA, mRNA transcriptions of the two inflammatory factors were assessed by Western Blot. Results： Curcuma wenyujin diterpenoid compound C within 10μg/mL and LPS within 10ng/mL had no effect on the proliferation of SGC-7901. Diterpenoid compound C significantly inhibited LPS-induced release of inflammatory factor IL-1β （P〈0.01） and increased the release of inflammatory depressive factor IL-2 （P〈0.01）. No significant difference was found in RT-PCR detection result. Curcuma wenyujin diterpenoid compound C inhibited the expression of P38, JNK and ERK protein in the MAPK pathway. Conclusion： Curcuma wenyujin diterpenoid compound C may inhibit LPS-induced release of inflammatory factors in gastric cancer cells, and its mechanism may be related to the inhibition of intracellular MAPK pathway.

关 键 词：温郁金二萜类化合物 细胞因子 内毒素 

分 类 号：R285.5[医药卫生—中药学]