槲皮素对溶菌酶淀粉样纤维化的抑制作用  

Inhibitory Effect of Quercetin on Amyloidogenesis of Lysozyme

作　　者：严向阳[1] 邢艳菲[1] 李文涓[1] 曾成鸣[1] 

机构地区：[1]陕西师范大学化学化工学院,西安710062

出　　处：《徐州工程学院学报（自然科学版）》2013年第2期79-84,共6页Journal of Xuzhou Institute of Technology(Natural Sciences Edition)

摘　　要：通过研究槲皮素抑制溶菌酶淀粉样纤维化及纤维的细胞毒性发现,槲皮素能够抑制溶菌酶的淀粉样纤维化,溶解成熟的溶菌酶纤维,将淀粉样纤维转化成为无定形沉积,抑制溶菌酶纤维导致的溶血作用.槲皮素的这种作用与槲皮素-蛋白间的疏水相互作用相关,而与槲皮素的抗氧化作用无关.In the present study the anti-amyloidogenic activity of quercetin has been investigated. The results showed that quercetin inhibited amyloid fibrillation of hen egg-white lysozyme and destabilized the preformed amyloid fibrils. Lysozyme fibrils were transformed into amorphous deposits upon treating the fi-brils with quercetin. As a consequence the β-sheet structure of the fibrils was disrupted and the fibrillar cy-totoxicity was attenuated. The anti-amyloidogenic effect of quercetin is not attributed to its anti-oxidative property but rather to its hydrophobic interaction with the fibrils.

关 键 词：槲皮素 溶菌酶 淀粉样纤维化 细胞毒性 疏水作用 

分 类 号：R284[医药卫生—中药学]