Nogo-A蛋白在大鼠心肌细胞中的表达变化及其在细胞缺氧性凋亡中的作用  

Expression of Nogo-A protein in rat cardiomyocytes and its effect on hypoxia-induced apoptosis

作　　者：程亮[1] 易蔚[1] 任凯[1] 裴建明[2] 

机构地区：[1]第四军医大学西京医院,西安710032 [2]第四军医大学生理学教研室

出　　处：《山东医药》2013年第22期13-15,21,共4页Shandong Medical Journal

基　　金：陕西省社发公关资助项目(2012K15-01-05)

摘　　要：目的探讨Nogo-A蛋白在心肌细胞中的表达及其在细胞缺氧性凋亡中的作用。方法①采用免疫组化法和免疫印记法测定成年大鼠心肌细胞(成熟心肌细胞)Nogo-A蛋白表达。②急性分离大鼠心肌细胞分为空白组、模型组及干预组,模型组及干预组建立心肌细胞缺氧模型,其中干预组制模前采用抗Nogo-A抗体干预。观察三组细胞凋亡情况、Nogo-A蛋白及Caspase-3和Caspase-12表达情况。结果①成熟心肌细胞:Nogo-A蛋白表达呈阳性。②缺氧心肌细胞:Nogo-A蛋白表达呈阳性;干预组及模型组均出现凋亡细胞,但干预组明显少于模型组;干预组及模型组Caspase-3和Caspase-12表达阳性率均明显高于空白组,但干预组明显低于模型组,P<0.05。结论心肌细胞中存在大量表达的Nogo-A蛋白;Nogo-A蛋白可能参与了心肌细胞的缺氧性凋亡。Objective To investigate the expression of Nogo-A protein in rat cardiomyocytes and its effect on hypoxia- induced apoptosis. Methods （1） Nogo-A protein expression was determined in adult rat cardiomyocytes by using immuno- histochemistry and Western blot. （2） The acutely isolated myocardial cells were divided into three groups： the control group, model group and intervention group. To establish the hypoxia-induced myocardial cell apoptosis models in the model group and intervention group, the intervention group was treated with the anti-Nogo-A antibodies before the modeling. The apoptosis, the expression of Nogo-A protein, Caspase-3 and Caspase-12 in three groups were observed. Results （1） Adult cardiomyocytes ： the expression of Nogo-A protein was positive. （2） Cardiomyocytes under hypoxia ： the expression of Nogo- A protein was positive; the intervention group and the model group appeared apoptotic cells, but the intervention group was significantly less than the model group; the positive rates of Caspase-3 and Caspase-12 expression were significantly higher in the intervention group and model group than those in the control group, but the intervention group was significantly lower than the model group, P 〈 0.05. Conclusions There was a large amount of expressed Nogo-A protein in myocardial cells. Nogo-A protein may be involved in the hypoxia-induced apoptosis of myocardial cells.

关 键 词：Nogo—A蛋白 心肌细胞 缺氧性凋亡 

分 类 号：R542.2[医药卫生—心血管疾病]