红花注射液保存人胎羊膜对大鼠皮肤切创愈合中MMP-2,MMP-9影响的研究  

Expressions of MMP-2,MMP-9 in Rat Skin Incised Wound Affected by Human Fetal Amniotic Preserved by Safflower Injection

作　　者：王君 罗秋燕[2] 边文玲[2] 申素芳[2] 

机构地区：[1]河南省新乡市红旗区人民医院,河南新乡453003 [2]新乡医学院第三附属医院

出　　处：《中国医学创新》2013年第18期1-3,共3页Medical Innovation of China

基　　金：河南省科技厅资助项目(编号:082102310084)

摘　　要：目的:研究经红花注射液保存后的人胎羊膜对大鼠皮肤切创愈合过程中基质金属蛋白酶(Matrix metalloproteinase-s,MMPS)-2,9表达的影响,探讨其促进切口愈合的机制,为其在临床的应用提供理论依据。方法:建立大鼠皮肤切创模型,观察切口愈合时间、感染率;在各时相点处死取材,应用免疫组织化学技术,通过计算机图像分析系统检测人胎羊膜对不同损伤时间大鼠皮肤组织中MMP-2,MMP-9的表达。结果:创后12h、1d、2d、3d,红花+羊膜组创缘周围MMP-2,MMP-9表达,较其他三组明显增强(P<0.05);红花+羊膜组感染率(5%)较其他三组明显减少(P<0.05)。结论:经红花注射液保存后的人胎羊膜在大鼠皮肤切创愈合过程中,可促进创缘周围MMP-2,MMP-9的表达;将其贴附创面能明显减少创口的感染率、促进创口的愈合。Objective： To observe the infection rate and MMP-2, MMP-9 expression in rat skin incised wound healing process with human fetal amnion preserved by the safflower injection. Explore the mechanism of skin healing process and reduce scar formation. Method： Rats were executed at different time after models of rats＇ skin incision were set, and then used immunohistochemical and auto image analysis system to detect the infection rate and MMP-2, MMP-9 expression in group of human amniotic membrane saved with safflower injection at different traumatic time intervals. Result： The expressions of MMP-2, MMP-9 was much higher in group of human amniotic membrane saved with safflower injection than others （ P〈0.05 ） ; the infection rate of the human amnion saved with safflower injection group （ 5% ） was less than that of the other groups （ P〈0.05 ） . Conclusion： The human amnion preserved by safflower injection could not only enhance the expression of MMP-2, MMP-9 around the area of injury, but also could reduce the infection rate of incised wound skin of rat, promote wound healing and reduce scar formation.

关 键 词：人胎羊膜 红花注射液 切创愈合 基质金属蛋白酶-2 基质金属蛋白酶-9 

分 类 号：R285.5[医药卫生—中药学]