Artificial Synthesis of TAT PTD-Tachyplesin Fusion Gene by Overlap Extension PCR  

作　　者：Daoshou QIU Xiaojin LIU Jun WANG Yongquan SU 

机构地区：[1]Crops Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Crop Genetics and Improvement of Guangdong Province [2]College of Ocean & Earth Sciences, Xiamen University

出　　处：《Agricultural Biotechnology》2013年第3期1-4,17,共5页农业生物技术（英文版）

基　　金：Supported by Natural Science Foundation of Guangdong Province(06025389);Post-doctoral Project of Fujian Province

摘　　要：This study aimed to investigate the synergism of the TAT PTD （ protein transduction domain in HIV-1 transactivator of transcription protein） to antibacte- rial peptide tachyplesin from Tachp/eus tr/dentatus. Treated with Pichia pastoris preferred codon optimization, using the eDNA sequence of tuchyplesin mature pep- tide （54 aa） harboring TAT FFD sequence （11 aa） as reference template, six single-stranded oligonueleotides were designed, the sequences of restriction sites EcoR I and Xba I were introduced to the 5＇ end of primers P1 and P6, respectively. TAT PTD ＋ Tachyplesin fusion gene with a full length of 219 bp was artificially synthesized by overlap extension PCR, which laid the preliminary foundation for subsequent functional and synergism studies.This study aimed to investigate the synergism of the TAT PTD （ protein transduction domain in HIV-1 transactivator of transcription protein） to antibacte- rial peptide tachyplesin from Tachp/eus tr/dentatus. Treated with Pichia pastoris preferred codon optimization, using the eDNA sequence of tuchyplesin mature pep- tide （54 aa） harboring TAT FFD sequence （11 aa） as reference template, six single-stranded oligonueleotides were designed, the sequences of restriction sites EcoR I and Xba I were introduced to the 5＇ end of primers P1 and P6, respectively. TAT PTD ＋ Tachyplesin fusion gene with a full length of 219 bp was artificially synthesized by overlap extension PCR, which laid the preliminary foundation for subsequent functional and synergism studies.

关 键 词：TAT protein transduetion domain （TAT PTD） TACHYPLESIN Overlap extension PCR 

分 类 号：S917.4[农业科学—水产科学]