Genetic Variation of Grey-rumped Pheasant (Torquatus group) Populations in China  

作　　者：Jiangyong QU 

机构地区：[1]Hainan Key Laboratory for Herpetological Research/Department of Life Sciences and Technology, Qiongzhou University [2]Department of Zoology and Entomology, Rhodes University

出　　处：《Agricultural Biotechnology》2013年第3期50-55,67,共7页农业生物技术（英文版）

基　　金：Supported by Natural Science Foundation of Hainan Province(NO:311055);Doctoral Start-up Research Fund of Qiongzhou University(No:QYXB201009);Co-operation Fund between University and Locality of Sanya City(No:2010YD22);Fund for Zoology Major Course of Hainan Province

摘　　要：The polymerase chain reaction combined with direct sequencing was employed to deduce the variety of the grey-rumped pheasant populations in China. Totally 1079 basepair sequences of mitochondrial DNA （mtDNA） control-region gene were analyzed from 121 grey-rumpod pheasants obtained from 13 Chinese lo- calities, 76 variable sites resulted in 91 haplotypes. These populations possessed a high level of genetic diversity, indicating that during their evolution effective population size and polymorphism were not reduced by climatic fluctuations in the Pleistocene ice ages. Phylogenetie analysis indicates that all populations were clustered into two groups, western and eastern. The degree of population differentiation presumably results from a low gene flow （ N, = 0. 33, F,, = 0. 60, P 〈 0. 001 ） between the two groups, and the divergence occurred in the years （2.1 ×10^5 - 2.2 × 105 ）. The genetic structure of grey-rumped pheasants in China ap- pears to be the result of the uplift of Qinghai-Tibet Plateau, the existence of Qinling Mountains and Liupan Mountains and cyclical climatic oscillations during the late Pleistocene.The polymerase chain reaction combined with direct sequencing was employed to deduce the variety of the grey-rumped pheasant populations in China. Totally 1079 basepair sequences of mitochondrial DNA （mtDNA） control-region gene were analyzed from 121 grey-rumpod pheasants obtained from 13 Chinese lo- calities, 76 variable sites resulted in 91 haplotypes. These populations possessed a high level of genetic diversity, indicating that during their evolution effective population size and polymorphism were not reduced by climatic fluctuations in the Pleistocene ice ages. Phylogenetie analysis indicates that all populations were clustered into two groups, western and eastern. The degree of population differentiation presumably results from a low gene flow （ N, = 0. 33, F,, = 0. 60, P 〈 0. 001 ） between the two groups, and the divergence occurred in the years （2.1 ×10^5 - 2.2 × 105 ）. The genetic structure of grey-rumped pheasants in China ap- pears to be the result of the uplift of Qinghai-Tibet Plateau, the existence of Qinling Mountains and Liupan Mountains and cyclical climatic oscillations during the late Pleistocene.

关 键 词：Genetic variation Grey-rumped Pheasants Mitochondrial DNA Population differentiation 

分 类 号：Q958[生物学—动物学] Q953