Q-开关激光对黄褐斑动物模型黑素细胞周期与凋亡的影响  

Biological effect of the 1064nm Q-switched Nd:YAG laser radiation on the epidermal melanocytes of Chloasma type guinea pigs experimental mode

作　　者：杨鹏[1] 杨慧兰[2] 麦跃[1] 戴勇[3] 孙林潮[1] 鲁东平[4] 

机构地区：[1]大连医科大学附属深圳阳光医院皮肤科,广东深圳518000 [2]南方医科大学附属广州军区广州总医院皮肤科,广东广州510010 [3]暨南大学医学院第二附属深圳市人民医院动物实验中心 [4]深圳市宝安区慢性病防治院

出　　处：《中国美容医学》2013年第12期1286-1291,共6页Chinese Journal of Aesthetic Medicine

基　　金：2010年广东省深圳市科学技术计划项目基金资助(深圳市科技计划项目编号:201003464)

摘　　要：目的:探讨Q-开关Nd:YAG激光不同能量照射黄褐斑动物模型对表皮黑素细胞周期与凋亡的影响。方法:用Q-开关激光不同能量照射黄褐斑动物模型,取表皮黑素细胞进行细胞培养,光学显微镜观察黑素细胞形态学变化,流式细胞仪检测照射后细胞周期与凋亡。结果:软件分析结果显示,除高能高频组外Q-开关Nd:YAG 1064nm激光第一次照射后即刻各能量密度与频率组黑素细胞形态、细胞周期与凋亡无明显改变。第5次激光照射后即刻,高能高频、高能低频和低能高频组与照射前比较树突数量明显减少和长度明显缩短,S期细胞比率减少,凋亡率显著增加,差异有统计学意义(P<0.01)。照射后1周各组数据更加显著,随后照射后3周又呈明显上升趋势。低能低频照射组照射前后无显著差异(P>0.01)。结论:当激光能量和频率达到一定阈值时能使黑素细胞体积变小、树突数量减少、长度缩短、S期细胞比率减少、凋亡率显著增加。为临床应用Q-开关1064nm Nd:YAG激光治疗色素性疾病提供了实验依据。Objective To investigate the biological effects of The different influence of Q-switched Nd：YAG 1064nm laser radiation on the epider malmelanocytes of Chloasma type guinea pigs experimental mode. Methods Four kinds of energy densities and frequency from Q-switched Nd： YAG 1064nm laser were used on the skin of Chloasma type guinea pigs experimental mode.The morphological changes of cells were observed by light microscopy. Then we used the flow cytometry to detect the cell cycle and apoptosis after irradiation. Results Software analysis results show,There was no noticeable alteration in the cell cycle,apoptosis and morphology of melanocytes after irradiation instantly by all different energy densities and frequency group besides high energy density and high frequency group. Compared with the preoperative,The high energy high frequency, high frequency low frequency and low energy high frequency group of the number of dendrites decreased and the length of them became shorter.,the percentage of cells in S-phase decreased, but the apoptosis rate increased significantly. Followed by the postoperative three weeks showing a rising trend. The low energy low frequency exposure group had no significant change before and after irradiation. Conclusion When the energy density and frequency achieves at specified value. Q- switched Nd：YAG 1064nm laser can result in the number of dendrites decreased and the length of them became shorter,decreased the cycle of melanocytes and promote the apoptosis of melanocytes. It is worth to study later.It make valid to make use of Q-switched Nd：YAG 1064nm laser for treatment of the pigmented skin diseases.

关 键 词：激光 黑素细胞 细胞周期 细胞凋亡 

分 类 号：R758.42[医药卫生—皮肤病学与性病学]