山东地区汉族人群15个X-STR基因座的遗传多态性  

Genetic Polymorphisms of 15 X-STR Loci in Shandong Han Population

作　　者：马腾[1] 徐婧[1] 杨亚军[1] 孙宽[2] 薛付忠[3] 金力[1] 李士林[1] 

机构地区：[1]复旦大学生命科学学院现代人类学教育部重点实验室,上海200433 [2]司法部司法鉴定科学技术研究所上海市法医学重点实验室,上海200063 [3]山东大学公共卫生学院,山东济南250012

出　　处：《法医学杂志》2013年第3期202-205,208,共5页Journal of Forensic Medicine

基　　金：国家高技术研究发展计划(863计划)(SS2012AA021303);国家自然科学基金面上项目(31071096)

摘　　要：目的研究山东地区汉族人群15个X-STR基因座的遗传多态性,建立法医学应用数据库。方法采用Primer Premier 5.0软件设计多重PCR引物,用4色荧光(FAM、VIC、NED、TET)进行标记,建立多重PCR体系,对山东地区无关汉族人群481例个体(女性295例,男性186例)的15个X染色体上筛选的STR基因座(DXS10011、DXS101、GATA165B12、DXS6795、DXS6800、DXS6801、DXS6803、DXS7132、DXS7133、DXS7423、DXS7424、DXS8377、DXS8378、DXS9898和HPRTB)进行检测。结果所检测的15个X-STR基因座中,GATA165B12、DXS6800、DXS6803、DXS7133与DXS7423在中国山东汉族人群中具有中度多态性,其余10个基因座都具有高度多态性(PIC>0.5,H>0.5)。群体中男性样本之间没有检测到共享单倍型。结论构建的荧光标记复合扩增体系为建立中国山东汉族人群X-STR基因座群体遗传学数据库及其法医学应用提供了有效的手段。Objective To investigate the genetic polymorphisms of 15 X-STR loci in Shandong Han pop- ulation in order to establish the forensic application database. Methods The multi-PCR primers of these loci were designed by Primer Premier 5.0 software and labeled by 4 fluoresceins （FAM, VIC, NED and TET）. The developed multi-PCR was used to investigate 15 X-STR loci （DXSIO011, DXSIO1, GATA165B12, DXS6795, DXS6800, DXS6801, DXS6803, DXS7132, DXS7133, DXS7423, DXS7424, DXS8377, DXS8378, DXS9898 and HPRTB） selected from the X chromosome of 481 unrelated individuals （295 females and 186 males） in Shandong Han population. Results Among the 15 X-STR loci, GATA165B12, DXS6800, DXS6803, DXS7133 and DXS7423 showed moderate polymorphisms, while the rest 10 X-STR loci showed high polymorphisms （PIC〉0.5 and H〉0.5）. No shared haplotype was detected among the males in Shandong Han population. Conclusion The developed multi-PCR system with fluorescence detection provides an effective way to establish X-STR loci database of population genetics in Shandong Hart population and shows its forensic application.

关 键 词：法医遗传学 多态现象 遗传 X—STR 山东 汉族 

分 类 号：DF795.2[医药卫生—法医学]