改良TRIzol法同步提取血液RNA和DNA  

Modified TRIzol Method for RNA and DNA Co-extraction from Blood

作　　者：秦娟娟[1] 路志勇[2] 焦章平[2] 朱晓君[2] 王颖希[1] 唐晖[2] 

机构地区：[1]山西医科大学基础医学院法医学系,山西太原030001 [2]北京市公安局刑侦总队,北京100192

出　　处：《法医学杂志》2013年第3期209-211,共3页Journal of Forensic Medicine

基　　金：北京市科技计划资助项目(Z111100056811024)

摘　　要：目的建立一种应用TRIzol试剂针对同一生物检材在提取总RNA的同时又能提取DNA的新方法。方法使用传统TRIzol法将含有总RNA的水相移除,调节中间层和有机相的pH值,乙醇沉淀DNA,运用DNA IQTMsystem试剂盒纯化DNA。检测DNA纯度和质量浓度,并以之为模板进行PCR-STR分型。与传统TRIzol法提取的基因组DNA进行比较。结果改良TRIzol法提取的基因组DNA较传统TRIzol法提取的基因组DNA纯度和质量浓度高,扩增后STR分型良好。结论改良TRIzol法可靠易行,能够实现同一样本同时提取RNA和DNA的目的,可以满足法庭科学个体识别和亲缘鉴定的要求。Objective To establish a new method for RNA and DNA coextraction from the same sam ple by TRIzol reagent. Methods After the aqueous phase which contained total RNA was removed by traditional TRIzol method, the values of pH of the interphase phase and organic phase were adjusted. The DNA was precipitated with ethanol and purified with DNA IQTM system. The purified DNA was measured in quality and quantity. As the template, it was amplified and typed by PCRSTR. The data was compared with that extracted by traditional TRIzol method. Results The DNA extracted by this modified method showed a better result of quality and quantity than that by traditional TRIzol method and a good STR typing. Conclusion The modified TRIzol method is advisable and reliable to simultane ously extract both DNA and RNA from the same sample. It could be used for individual identification and paternity testing to satisfy the need of forensic science.

关 键 词：法医遗传学 提取法 TRIZOL法 血液 

分 类 号：DF795.2[医药卫生—法医学]