HPLC测定盆炎净片中芍药苷的含量  

Content Determination of Paeoniflorin in Penyanjing Tablets by HPLC

作　　者：袁兆坤 李亚红[2] 王秀[3] 

机构地区：[1]安徽华康医药集团有限公司,安徽宿州234000 [2]合肥市第二人民医院,安徽合肥230011 [3]皖南医学院,安徽芜湖241000

出　　处：《现代中药研究与实践》2013年第3期63-65,共3页Research and Practice on Chinese Medicines

摘　　要：目的建立盆炎净片中芍药苷的含量测定方法。方法采用HPLC法,十八烷基硅烷键合硅胶色谱柱(6.0mm×150mm,5μm);以乙腈-水(13:87)为流动相,流速1.0mL.min-1;检测波长230nm。结果芍药苷在0.155 2μg.mL-1～1.552μg.mL-1的范围内与峰面积积分值呈良好的相关性,其线性回归方程为:Y=137 002X+12 458,r=0.999 9;平均加样回收率为99.1%(n=6,RSD%=1.01%)。结论实验表明,该法操作简便、灵敏度高、重现性好,利于盆炎净片的质量控制。Objective To develop an HPLC method for determination of the content of Paeoniflorin in Penyanjing tablets. Methods Using the method of HPLC with Octadecyl silane bonded silica column （ 6.0mm × 150mm, 5μm ）; The mobile phase consisted of acetonitrile-water （ 13：87 ）, the flow rate was 1.0ml· min 1; The detection wavelength was at 323 nm. Results There was a good linear relationship between the concentration of Paeoniflorin and peak area value when the concentrations of Paeoniflorin was within the range of 0.155 2μgomL^-1-1.552μg-mL^-1 .Equation of linear regression： Y= 137 002X＋12 458,1-=0.999 9; the average recovery was 99.1% （n=6,RSD%=1.01%）. Conclusion The experiment shows that the method has the advantages of simple operation, high sensitivity, good repeatability. It would be used in quality control of Penyanjing tablets.

关 键 词：HPLC 盆炎净片 芍药苷 含量测定. 

分 类 号：R927.2[医药卫生—药学]