Determination of sildenafil by preconcentration on surfactant coated polymeric resin followed by spectrofluorimetry  

作　　者：Chien C.Wang Roxana A.Gómez Liliana P.Fernandez 

机构地区：[1]INQUISAL-CONICET,Chacabuco y Pedernera,5700-San Luis,Argentina [2]Departamento de Farmacia,Facultad de Química,Bioquímica y Farmacia,Universidad Nacional de San Luis,Argentina [3]rea de Química Analítica,Facultad de Química,Bioqímica y Farmacia,Universidad Nacional de San Luis,Argentina

出　　处：《Journal of Pharmaceutical Analysis》2013年第3期173-179,共7页药物分析学报（英文版）

基　　金：INQUISAL-CONICET (Instituto de Química de San Luis-Consejo Nacional de Investigaciones Científicas y Tecnológicas);FONCYT (Fondo Nacional de Ciencia y Tecnología);the National University of San Luis (Projects SECyT-UNSL 22/Q828 and PIP-CONICET 11220100100405) for their financial support

摘　　要：The illicit addition of phosphodiesterase type-5 （PDE-5） inhibitors like sildenafil （Viagra） in product offered as herbal medicine or dietary supplement for male erectile dysfunction has concerned authorities in recent times. In this paper, we proposed a sensitive surfactant-coated Amberlite XADTM resin for sildenafil preconcentration method with spectrofluorimetric detection. Retention capacity of micellar coated XAD resin for sildenafil was studied and the obtained eluate was measured by spectrofluorometer at excitation and emission wavelengths of 350 and 430 nm, respectively. This method allowed the detection of sildenafil at 0.15 ng/mL with linear range of 0.0003-7.0 μg/mL. The method has been successfully applied to the analysis of some local commercially available herbal medicines and urine.The illicit addition of phosphodiesterase type-5 （PDE-5） inhibitors like sildenafil （Viagra） in product offered as herbal medicine or dietary supplement for male erectile dysfunction has concerned authorities in recent times. In this paper, we proposed a sensitive surfactant-coated Amberlite XADTM resin for sildenafil preconcentration method with spectrofluorimetric detection. Retention capacity of micellar coated XAD resin for sildenafil was studied and the obtained eluate was measured by spectrofluorometer at excitation and emission wavelengths of 350 and 430 nm, respectively. This method allowed the detection of sildenafil at 0.15 ng/mL with linear range of 0.0003-7.0 μg/mL. The method has been successfully applied to the analysis of some local commercially available herbal medicines and urine.

关 键 词：SILDENAFIL Herbal medicines Spectrofluorimetricanalysis SURFACTANT 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.3[化学工程—制药化工]