急性缺氧对大鼠远端肺静脉平滑肌细胞内钙浓度的影响  

Effect of acute hypoxia on intracellular calcium cation concentration in rat distal pulmonary venous smooth muscle cells

作　　者：彭公永[1] 周玉民[1] 蒋永亮[1] 胡国平[1] 邹威凤[1] 

机构地区：[1]广州医学院第一附属医院广州呼吸疾病研究所呼吸疾病国家重点实验室,广州510120

出　　处：《解剖学杂志》2013年第3期305-308,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金（81000020）;羊城学者科研计划学术骨干项目（10A025G）;广州市属高校科研项目（10A276）;呼吸疾病国家重点实验室青年科学基金支持项目（11）

摘　　要：目的：研究急性缺氧对大鼠远端肺静脉平滑肌细胞（PVSMC）细胞内钙浓度（[Ca^2＋]i）的影响及L型电压依赖性钙通道（VDCC）阻断剂硝苯地平的作用，为缺氧性肺动脉高压发病机制的进一步研究提供理论依据。方法：胶原酶消化法培养大鼠远端PVSMC，利用荧光显微镜和细胞内钙浓度检测系统观测急性缺氧（4％O2）、高钾（60mmol／LKCl）溶液对PVSMC的[Ca^2＋]i影响及硝苯地平的干预作用。结果：对照组PVSMC的[Ca^2＋]i随时间变化维持基线水平；缺氧组PVSMC急性缺氧后，[Ca^2＋]i迅速升高并维持平台水平，△[Ca^2＋]i达82．83nmol／L＋23．03nmol／L；硝苯地平干预组PVSMC予急性缺氧和5μmol／L硝苯地平干预后，[Ca^2＋]i升高幅度较小；高钾溶液孵育PVSMC后，[Ca^2＋]i迅速增高，5μmol／L硝苯地平能完全阻断PVSMC的[Ca^2＋]i对高钾溶液的反应。结论：急性缺氧可使大鼠远端PVSMC的[Ca^2＋]i升高，其机制可能与激活PVSMC的VDCC和另外的非VDCC依赖的钙通道导致细胞外Ca^2＋内流有关。Objective： To study the effect of acute hypoxia on intracellular calcium cation concentration （[Ca^2＋]i） in rat distal pulmonary venous smooth muscle cells （PVSMC） and the role of the L-type voltage-dependent Ca^2＋ channels （VDCC） antagonist nifedipine. Methods： The rat distal PVSMC were cultured by collagenase digestion. The effects of acute hypoxia （4% 02 ） and high KC1 （60 mmol/L） on [Ca^2＋]i in PVSMC and the role of nifedipine were tested by fluorescence microscopy and InCyte [Ca^2＋ ]i measurement system. Results： The time course of [Ca^2＋ ]i in control PVSMC was stable at baseline. Acute hypoxia induced a marked increase in [Ca^2＋ ]i in PVSMC that rose quickly to a peak of/k [Ca^2＋ ]i =82.83＋23.03 nmol/L and followed by a plateau. 5μmol/L nifedipine partially attenuated the [Ca^2＋ ]i response to acute hypoxia and the peak of/k [Ca^2＋ ]i averaged 42.17±6.18 nmol/L in PVSMC. 60 mmol/L KC1 caused a quick increase in [Ca^2＋ ]i in PVSMC with the peak of/k [Ca^2＋ ]i = 183. 03 ~ 37. 84 nmol/L and 5 μmol/L nifedipine completely blocked the [-Ca^2＋ ]i response to KC1 in PVSMC. Conclusion： Acute hypoxia increased the [Ca^2＋ ]i in rat distal PVSMC. An increase in [Ca^2＋]μ induced by acute hypoxia may the related to the influx of Ca^2＋ through VDCC and another VDCC-independent Ca^2＋ channels in PVSMC.

关 键 词：急性缺氧 细胞内钙浓度 肺静脉平滑肌细胞 L型电压依赖性钙通道 大鼠 

分 类 号：R135.2[医药卫生—劳动卫生]