南瓜基因组DNA的细菌人工染色体(BAC)文库的构建  

BAC Library Construction for Pumpkin Genome

作　　者：赵茜[1] 张丽艳[2] 徐丽珍[1] 吴建忠[1] 

机构地区：[1]黑龙江省农业科学院经济作物研究所,黑龙江哈尔滨150086 [2]中国科学院北方粳稻分子育种联合研究中心,黑龙江哈尔滨150086

出　　处：《安徽农业科学》2013年第11期4738-4739,4744,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]构建南瓜基因组DNA的细菌人工染色体(BAC)文库。[方法]以南瓜幼芽为材料,利用HindⅢ酶切体系,初步构建南瓜基因组DNA的BAC文库。[结果]研究成功构建了南瓜基因组DNA的BAC文库。[结论]该技术为南瓜相关基因的克隆、功能验证、物理图谱的构建和基因组测序等研究工作奠定了基础。[Objective] The paper was to construct the bacterial artificial chromosome（BAC） library for pumpkin genomic DNA.[Method] The BAC library of pumpkin genomic DNA was constructed by using pumpkin buds as materials and HindIII enzyme-cutting system.[Result] The BAC Library of pumpkin genomic DNA was constructed successfully.[Conclusion] The technology laid a foundation for the cloning and functional verification of related genes,physical mapping and genome sequencing of pumpkin.

关 键 词：南瓜 基因组 BAC 

分 类 号：S642.1[农业科学—蔬菜学]