TL_2的鉴定及其对植物病原菌的抑制作用  

Identification of TL_2 and the Inhibitive Effect of TL_2 to Fungal Pathogens

作　　者：赵晓燕[1] 洪永聪[2] 郭凯[1] 张新建[1] 周红姿[1] 李纪顺[1] 杨合同[1] 

机构地区：[1]山东省科学院中日友好生物技术研究中心/山东省应用微生物重点实验室,山东济南250014 [2]武夷学院茶学院茶学与生物系,福建南平354300

出　　处：《福建农业学报》2013年第4期353-356,共4页Fujian Journal of Agricultural Sciences

基　　金：山东省科技攻关项目(2010GSF10206;2012GSF12114);山东省科学院博士基金项目(201207-18)

摘　　要：对枯草芽孢杆菌TL2进行了16SrDNA序列分析,结合菌落形态、生理生化特征研究,将其鉴定为芽孢杆菌属的枯草芽孢杆菌Bacillus subtilis。通过对峙培养法测定TL2对10种植物病原菌的抑制作用,结果表明,对峙培养5d后,TL2对10种病原菌均有不同程度的抑制作用,其中枯草芽孢杆菌TL2对辣椒疫霉菌的平均抑制率最高(65.12%),对苹果轮纹病菌的平均抑制率最低(24.14%)。Based on morphological, physiological and biochemical characteristics, combined with analysis of 16S rDNA sequences, the strain TL2 was identified as Bacillus subtilis. The inhibitive efficacy of Bacillus subtilis TL2 to ten kinds of fungal pathogens were tested by dual culture. The result showed that Bacillus subtilis TL2 has significant inhibition to ten pathogenic fungi after 5d with dual culture. The best average inhibitive rate of Bacillus subtilis TL2 to Phytophthora capsici was 65.12% and the lowest average inhibitive rate of Bacillus subtilis TL2 to Botrvosphaeria dothidea was 24.14 %.

关 键 词：枯草芽孢杆菌TL2 16S RDNA序列 植物病原菌 平均抑制率 

分 类 号：S476[农业科学—农业昆虫与害虫防治] Q93-331[农业科学—植物保护]