利用微卫星多态性分析白羽半番鸭亲本种群群体遗传结构  

Analysis on Genetic Structure of Parental Populations of White-plumage Mule Duck by Microsatellite Polymorphism

作　　者：缪中纬[1] 段园园[2] 郑嫩珠[1] 朱志明[1] 辛清武[1] 陈晖[1] 

机构地区：[1]福建省农业科学院畜牧兽医研究所,福建福州350013 [2]福建省农业科学院农业经济与科技信息研究所,福建福州350003

出　　处：《福建农业学报》2013年第5期419-422,共4页Fujian Journal of Agricultural Sciences

基　　金：福建省种业创新与产业化工程项目(2011FJZY-9-02);现代农业产业技术体系建设专项资金(nycytx-45-06)

摘　　要：应用8个微卫星标记对5个鸭种群进行遗传多样性检测。利用等位基因频率计算各群体的基因杂合度、多态信息含量及群体间的遗传距离,并根据遗传距离进行聚类分析。结果表明:8个微卫星标记座位平均有效等位基因数为2.888,5个鸭群的平均基因杂合度为0.592～0.658,平均多态信息含量为0.422～0.598,为中高度多态。各母本种群间的遗传距离0.187～0.445,番鸭与4个母本的遗传距离0.322～0.445。从聚类分析结果来看,中型母本与小型母本和大型母本先聚为1类,再与M18母本相聚,最后与番鸭相聚。The genetic polymorphism of five duck breeds was detected by eight microsatellite markers. Genetic heterozygosity, polymorphic information content and genetic distance were calculated by allele frequency, and the cluster analysis was based on genetic distance. The result showed that the average effective allele number per locus was 2. 888 at eight microsatellite locus, the average genetic heterozygosity was 0. 592-0. 658, and the average polymorphic information content was 0. 422-0. 598 in the five duck populations. The genetic distance was 0. 187- 0. 445 among the female parents, and the genetic distance was 0. 322 -0. 445 between muscovy duck and the four female parents. The cluster analysis result showed that medium-sized female parent with mini-sized and large-sized clustered together firstly, and then clustered with M18 female parent, finally clustered with Muscovy duck.

关 键 词：半番鸭亲本 微卫星多态性 遗传结构 

分 类 号：S831.2[农业科学—畜牧学]