黑色素基因在半番鸭及番鸭不同羽色中的差异表达研究  

Differential Expressions of Melanin Genes in the Mule and Muscovy Duck with Different Plumage Colors

作　　者：郑嫩珠[1] 辛清武[1] 缪中纬[1] 朱志明[1] 陈晖[1] 

机构地区：[1]福建省农业科学院畜牧兽医研究所,福建福州350013

出　　处：《福建农业学报》2013年第5期427-431,共5页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划项目--省属公益类科研院所基本科研专项(2010R1025-6);福建省自然科学基金项目(2010J01107);福建省种业创新与产业化工程建设项目(2011FJZY-9-02)

摘　　要：黑色素对动物毛(羽)色的形成具有重要作用。为探寻半番鸭和番鸭MC1R和TYR基因表达对其羽色的遗传效应,采用实时荧光定量PCR技术分析MC1R和TYR基因在半番鸭和番鸭不同羽色皮肤组织中的表达情况。结果显示:MC1R和TYR基因在半番鸭和番鸭黑羽皮肤中的mRNA表达量均极显著高于白羽皮肤(P<0.01),MC1R基因在半番鸭和番鸭黑羽皮肤中的表达量分别为白羽皮肤的9.08倍和3.13倍,TYR基因在半番鸭和番鸭黑羽皮肤中的表达量分别为白羽皮肤的2.50和14.54倍。由此说明,TYR和MC1R基因表达水平与羽色形成有一定的关系。Melanin plays important roles in the formation of animal hair and plumage color. Previous studies have indicated that genes of melanocortin 1-receptor （MCIR） and tyrosinase （TYR） are involved in the synthesis of melanin. In order to examine genetic effects of expressions of MC1R and TYR on plumage color, the expression levels of MC1R and TYR in different plumage color skins of mule and muscovy duck were analyzed using quantitative real-time PCR. The results showed that the expression of TYR and MC1R in black plumage skins of both mule and muscovy duck were extremely significantly （P（0.01） higher than that in white plumage skins. The expressions of MC1R in black plumage skins of mule duck and muscovy duck were 9.08 and 3.13 times respectively higher than that in white plumage skin, while the expressions of TYR in black plumage skin of mule duck and muscovy duck were 2.5 and 14.54 times respectively higher than that in white plumage skin. These datas suggested that expression levels of TYR and MCIR were related with the forming of plumage color.

关 键 词：半番鸭 番鸭 羽色 黑色素基因 实时荧光定量PCR 

分 类 号：S834[农业科学—畜牧学]