原花青素对反式脂肪酸染毒小鼠精子质量的影响  

Effect of procyanidins on reproductive toxicity induced by Tans Fatty Acids in male mice

作　　者：凌娅[1] 王友良 邹学敏[3] 文静[1] 李东阳[1] 

机构地区：[1]南华大学公共卫生学院 [2]湖南环境生物技术学院,湖南衡阳421001 [3]长沙医学院,长沙410219

出　　处：《微量元素与健康研究》2013年第4期1-3,共3页Studies of Trace Elements and Health

基　　金：衡阳市科技局基金资助(2005-16)

摘　　要：目的:探讨原花青素(PC)对反式脂肪酸(TFA)致雄性小鼠精子质量的影响。方法:50只成年昆明种(KM)雄性小鼠,随机分为5组:溶剂对照组、TFA染毒组、低、中、高剂量PC保护组。溶剂对照组灌胃生理盐水;TFA染毒组按100 mg/kg剂量灌胃TFA;3个保护组分别按25.0 mg/kg、50.0 mg/kg、100.0 mg/kg的剂量灌胃PC。除溶剂对照组灌胃等体积的生理盐水外,其它每组均上午灌胃TFA、下午灌胃PC,时间90 d。实验结束后取血后处死动物,测定相关指标。结果:与对照组比较,TFA染毒组小鼠精子数量、精子存活率、精子活力均降低,畸形率增加(P<0.05);中、高剂量PC保护组小鼠精子数量、精子存活率、精子活力均有不同程度的增加,畸形率减少与TFA染毒组比较差别有显著性(P<0.05),精子畸形以尾折叠、卷尾、尾体弯曲、不规则精子、双尾精子为主;TFA染毒组SOD降低,MDA升高;PC保护后SOD升高,MDA降低与染毒组比较差别有显著性(P<0.05)。结论:TFA对雄性小鼠精子质量有损伤作用;PC对小鼠精子损伤有保护修复作用,其机制与抗氧化损伤有关。Objective： To explore the effect of procyanidins on sperm injury induced by trans fat in male mice. Methods： 50 adult Kunming （KM） mice were randomly divided into 5 groups： solvent control group, TFA group, TFA＋ lowdose PC group, TFA＋ middose PC group, TFA＋ highdose PC group. Solvent control group was treated with equal volume of physiological saline; TFA group was treated with TFA intragastric administration at the dose of 100 mg/kg- TFA ＋ low, mid, high - dose PC groups were treated with TFA intragastric administration at the dose of 100 mg/kg and PC at the doses of 25.0mg/q,,g, 50.0 mg/kg, 100.0 mg/kg, respectively; all the treatment persisted for 90 days. Result：Compared with the control group, mouse sperm count, sperm survival rate and sperm survival activity decreased, while abnormal rate increased （P 〈 0.05 ）. In mid and high dose of PC treated group, adverse effect aforementioned was effectively inversed. Conclusion： Procyanidins （PC） have protection on sperm injury induced by TFAicarbazide （TFA） ; the mechanism may be related to alteration of the state of TFA induced oxidative damage.

关 键 词：反式脂肪酸 原花青素 生殖毒性 氧化损伤 

分 类 号：O625.31[理学—有机化学]