大豆凝集素le2基因RNA干扰表达载体构建及转化的研究  

Construction of RNAi Expressed Vector of Soybean Agglutinin le2 Gene and Transform Research

作　　者：魏强[1] 李鲁华[1] 王春艳[1] 付永平[1] 马建[1] 曲静[1] 王丕武[1] 

机构地区：[1]吉林农业大学农学院,吉林长春130118

出　　处：《大豆科学》2013年第3期306-309,共4页Soybean Science

摘　　要：以构建RNA干扰表达体系,探索改良大豆品质方法为目的,根据GenBank中已知的大豆凝集素le2基因核酸保守序列(AY342212),设计相应引物,克隆le2基因核心保守序列,测序并与原序列比对同源性为99.77%。以质粒p3301-PFNZ-α'作为基础载体,通过亚克隆构建含有RNA干扰元件和bar基因的表达载体pCAMBIA3301-le2RNAi。通过农杆菌转化法将RNA干扰表达载体导入受体大豆吉农28,获得PCR阳性T1代植株5株,种子23粒。研究结果为大豆品质改良奠定了基础。For the purpose of constructing RNAi expressed vector, and discussing the method to improve soybean quality, ac- cording to known conserved sequence（AY342212） of soybean agglutinin le2 gene in GenBank, right primer was designed and the conserved sequence was cloned. And the homology was 99.77% contrast to the original sequence. Herein, using P3301-PF- NA-α＇as the basic vector, the expression vector pCAMBIA3301-le2RNAi was constructed which contained RNA interference vector and bar gene through subcolone. The RNA interference expression vector was then transformed into cotyledon nodes of soybean Jinong 28 by Agrobacterium-mediated method. 5 positive transgenic plants in T1 generation were confirmed by PCR de- tection and 23 seeds were harvested from T1 transgenic plants. The results provided a basis for soybean quality improvement.

关 键 词：RNA干扰 大豆凝集素 载体构建 遗传转化 

分 类 号：S565.1[农业科学—作物学]