N-三甲基壳聚糖包衣PLGA纳米粒疫苗载药系统增加抗原交叉递呈的初步研究  

Effect of N-trimethyl Chitosan-coated PLGA Nanoparticles on Antigen Cross-presentation

作　　者：韩瑞玲[1] 郭咸希[2] 岳源[1] 丁虹[1] 

机构地区：[1]组合生物合成与新药发现教育部重点实验室,武汉大学药学院,武汉430071 [2]武汉大学人民医院药剂科

出　　处：《中国药师》2013年第6期794-797,共4页China Pharmacist

基　　金：中央高校基本科研业务费专项资金(编号:20420111134)

摘　　要：目的:探讨N-三甲基壳聚糖包衣聚乳酸-羟基乙酸共聚物[poly(lactic-co-glycolic acid),PLGA]纳米粒对诱导树突细胞交叉递呈的机制。方法:复乳法制备PLGA纳米粒并用N-三甲基壳聚糖(N-trimethyl chitosan,TMC60)进行包衣,分别包载模型抗原卵清白蛋白、异硫氰酸荧光素(fluorescein isothiocyanate,FITC)标记的卵清白蛋白(FITC-OVA)。将TMC60包衣和未包衣的纳米粒分别作用于体外培养的小鼠骨髓系树突细胞(murine bone marrow-derived dendritic cell,BMDC),用流式细胞仪检测BMDC对纳米粒子的吞噬动力学及BMDC表面分子CD83,MHCI和MHCII的表达;B3Z T细胞检测纳米粒被BMDC摄取后引起的交叉递呈反应。结果:TMC60包衣PLGA纳米粒可以促进BMDC吞噬作用;促进BMDC表达表面分子CD83和MHC I;增强BMDC对纳米粒包载抗原的交叉递呈作用。结论:TMC60包衣PLGA纳米粒可增强BMDC对外源性抗原的摄取及交叉递呈作用。Objective：To investigate the effect of N-trimethy chitosan（TMC60）-coated PLGA nanoparticles on cross-presentation of murine bone marrow-derived dendritic cells（BMDC）.Method;PLGA nanoparitlces were prepared by W1/O/W2 method.Ovalbumin （OVA） or FITC labeled OVA（FITC-OVA） as the model antigen was encapsulated in PLGA nanoparticles.TMC60 were used to coat PLGA nanoparticles.The prepared nanoparticles were used to stimulate BMDC.Flow cytometry was used to analyze the uptake kinetics of the nanoparticles and the expression of surface molecular CD83,MHCⅠand MHCⅡon BMDC.B3Z T cell was used to detect cross-presentation.Result：Compared with that of OVA-NPs group,BDMC stimulation of OVA-NPs/TMC60 group led to the increase in endocytosis and up-regulation of CD83 and MHC I.Furthermore,OVA-NPs/protamine-treated BMDC also showed an enhanced cross-presentation to B3Z T cell hybridoma in vitro.Conclusion：TMC60 - coated PLGA nanoparticles can enhance the uptake and cross - presentation of encapsulated exogenous antigen.

关 键 词：N-三甲基壳聚糖 聚乳酸-羟基乙酸共聚物 纳米粒 交叉递呈 

分 类 号：R965.1[医药卫生—药理学]