HPLC法测定复脉灵胶囊中盐酸麻黄碱及盐酸伪麻黄碱含量  

Determination of Ephedrine Hydrochloride and Pseudoephedrine Hydrochloride in Fumailing Capsules by HPLC

作　　者：叶莎[1] 李兴华[2] 任欣欣[3] 马小亚[2] 雷建林[2] 李万静[3] 段宗明[3] 

机构地区：[1]西安交通大学医学院第二附属医院干保科,西安710004 [2]西安交通大学医学院第二附属医院药剂科 [3]西安交通大学医学院第二附属医院心内科

出　　处：《中国药师》2013年第6期841-843,共3页China Pharmacist

摘　　要：目的:建立复脉灵胶囊中盐酸麻黄碱、盐酸伪麻黄碱的含量测定方法。方法:采用HPLC法,流动相:乙腈-水-磷酸-三乙胺(3:97:0.1:0.1);色谱柱:Aglient TC-C_(18)(250 mm×4.6 mm,5μm);检测波长:205 nm;流速:1.2 ml·min^(-1);柱温:室温结果:盐酸麻黄碱的进样量在0.187～0.937μg范围内具有良好的线性关系(r=0.999 7),平均回收率为99.26%,RSD为2.70%(n=6);盐酸伪麻黄碱的进样量在0.226～1.130μg范围内具有良好的线性关系(r=0.999 7),平均回收率为98.77%,RSD为2.21%(n=6)。结论:该方法操作简便、灵敏度高、结果准确,可作为复脉灵胶囊中盐酸麻黄碱、盐酸伪麻黄碱的含量测定方法。Objective：To establish a method for the determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Fumailing capsules.Method：Ephedrine hydrochloride and pseudoephedrine hydrochloride in the capsules was determined by HPLC on an Aglient TC-C18 column（250 mm×4.6 mm,5μm）,the mobile phase consisted of acetonitrile-water-phosphoricacid-triethylamine （3：97：0.1：0.1） with a flow rate of 1.2 ml·min-1,the detection wavelength was 205 nm and the column temprature was room temprature. Result：The content of ephedrine hydrochloride had a good linearity within the range of 0.187-0.937μg（r=0.999 7）,the average recovery was 99.26%and RSD was 2.70%（n=6）.The content of pseudoephedrine hydrochloride had a good linearity within the range of 0.226-1.130μg（r=0.999 7）,the average recovery was 98.77%and RSD was 2.21%（n=6）.Conclusion：The method is simple,reproducible and accurate,and it can be used in the content determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Fumailing capsules.

关 键 词：复脉灵胶囊 盐酸麻黄碱 盐酸伪麻黄碱 高效液相色谱 

分 类 号：R927.2[医药卫生—药学]