蔗糖分子印迹聚合物的制备及表征  

Preparation and characterization of molecularly imprinted polymers of sucrose

作　　者：宁春园[1] 蒋林斌[1] 袁霞[1] 梁碧超[2] 叶少峰[2] 张永骏[1] 

机构地区：[1]广西大学化学化工学院,广西石化资源加工及过程强化技术重点实验室,广西南宁530004 [2]广西大学实验设备处,广西南宁530004

出　　处：《广西大学学报（自然科学版）》2013年第3期521-526,共6页Journal of Guangxi University（Natural Science Edition）

基　　金：广西自然科学基金资助项目(桂科自2012GXNSFAA053028);广西亚热带生物资源保护利用重点实验室基金项目(SB0804);广西大学科研基金项目(X081089)

摘　　要：分子印迹聚合物吸附选择性好,有利于印迹分子的分离纯化。以蔗糖为模板分子,甲基丙烯酸为功能单体,N,N'-亚甲基双丙烯酰胺为交联剂制备了蔗糖分子印迹聚合物,利用单因素试验考察了不同引发剂、引发剂用量、模板分子与功能单体的用量比、反应温度、反应时间、功能单体与交联剂的用量比对印迹聚合物吸附容量的影响,并用傅立叶变换红外光谱仪(FT-IR)和扫描电子显微镜(SEM)对印迹聚合物的结构进行了分析。实验结果表明,最佳反应条件为:引发剂为过硫酸铵,其浓度为1.2 mg/mL,反应时间18 h,反应温度50℃,甲基丙烯酸与蔗糖的用量比为4∶1,交联剂与甲基丙烯酸的用量比为5∶1。扫描电镜图显示,分子印迹聚合物具有纵横交错的网络和许多特异性识别孔穴。红外光谱表明,印迹聚合物中的模板分子能被很好地洗脱下来。The molecularly imprinted polymers have been discovered to have good selectivity for absorption. It is beneficial to separation and purification of the imprinted molecule. The molecularly imprinted polymers of sucrose were prepared by using the sucrose as template molecule, methyl methaerylate as functional monomer and N, N＇-methylene bisacrylamide as crosslinker. The effects of the different initiators, dosage of initiator, dosage ratio of sugar and methyl methacrylate, reaction temperature, reaction time and the dosage ratio of functional monomer and crosslinking agent on the adsorption capacity of molecularly imprinted polymers were investigated with single factor method. The structure of imprinted polymers was analyzed by IR, SEM. The experiments showed that the optimal reaction conditions were ammonium persulfate as initiator, its concentration 1.2 mg/mL, the reaction time in 18 h, the reaction temperature at 50℃, the dosage ratio of methyl methacrvlata and sugar in 4： 1, and the dosage ratio of crosslinking agent and methyl methacrylate in 5： 1. The SEM results showed that the molecularly imprinted polymers surface appeared crisscross network and many specific identification cavities. The FF-IR results showed that the template molecules in polymer were well eluted down.

关 键 词：蔗糖 甲基丙烯酸 N N’-亚甲基双丙烯酰胺 分子印迹聚合物 吸附容量 

分 类 号：O629.12[理学—有机化学]