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作 者:江燕[1] 虞国芬 张艳[1] 费冬[1] 徐婧[1]
机构地区:[1]杭州市余杭区第一人民医院妇产科,杭州311100
出 处:《南昌大学学报(医学版)》2013年第3期16-18,共3页Journal of Nanchang University:Medical Sciences
摘 要:目的探讨妊娠期肝内胆汁淤积症(ICP)胎盘组织中细胞凋亡及caspase-3的表达情况。方法以临产前行剖宫产术终止妊娠的ICP患者为ICP组(20例),同期因骨盆、胎位、社会因素等原因临产前行剖宫产术终止妊娠的健康孕妇为对照组(20例)。采用末端脱氧核苷酸转移酶介导、地高辛标记的dUTP缺口末端标记法(TUNEL)检测胎盘组织中合体滋养细胞、细胞滋养细胞、蜕膜细胞及绒毛间质细胞的凋亡情况,采用免疫组织化学法检测胎盘组织caspase-3蛋白表达水平。结果 ICP组胎盘组织中各细胞的凋亡率指数、caspase-3蛋白阳性表达率均高于对照组(P<0.05)。结论 ICP胎盘组织存在细胞凋亡以及凋亡相关蛋白表达增多的改变,ICP胎盘功能减退与细胞凋亡密切相关,细胞凋亡在ICP疾病的发生、发展中起重要作用。Objective To explore the apoptosis and caspase-3 expression in the placental tissue of patients with intrahepatic cholestasis of pregnancy(ICP).Methods Twenty ICP patients who underwent cesarean section to terminate the pregnancy before labor were selected as the ICP group,and 20 normal pregnant women who underwent cesarean section due to pelvis,fetal position or social factors as the control group.The apoptosis of syncytiotrophoblast,cytotrophoblast,decidual and interstitial cells was detected by TUNEL assay.The expression of caspase-3 protein was measured by immunohistochemistry.Results Compared with control group,the apoptosis rate and caspase-3 expression in placental tissue significantly increased in ICP group(P0.05).Conclusion The apoptosis and apoptosis-related protein expression increase in placenta of ICP patients.The ICP-induced placental insufficiency is closely related to the apoptosis,which plays an important role in the occurrence and development of ICP.
关 键 词:妊娠期肝内胆汁淤积症 胎盘 凋亡 CASPASE-3
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