Combining Solvent Extraction and Bioremediation for Removing Weathered Petroleum from Contaminated Soil  

作　　者：WU Guo-Zhong F.COULON YANG Yue-Wei LI Hong SUI Hong 

机构地区：[1]Graduate School at Shenzhen, Tsinghua University [2]School of Chemical Engineering and Technology, Tianjin University [3]Department of Environmental Science and Technology, School of Applied Sciences,Cranfield University [4]National Engineering Research Centre for Distillation Technology

出　　处：《Pedosphere》2013年第4期455-463,共9页土壤圈（英文版）

基　　金：Supported by the National High Technology Research and Development Program (863 Program) of China (No. 2009AA063102);the Program for Changjiang Scholars and Innovative Research Team in University, China (No. IRT0936);the Tianjin Municipal Natural Science Foundation of China (No. 12JCQNJC05300);the China Postdoctoral Science Foundation (No. 2013M530641);the National Natural Science Foundation of China (No. 41201497)

摘　　要：This study aimed to evaluate the efficacy, practicality and sustainability of a combined approach based on solvent extraction and biodegradation to remediate the soils contaminated with high levels of weathered petroleum hydrocarbons. The soils used in this study were obtained from the Shengli Oilfield in China, which had a long history of contamination with high concentrations of petroleum hydrocarbons. The contaminated soils were washed using a composite organic solvent consisting of hexane and pentane （4：1, v/v） and then bioremediated in microcosms which were bioaugmentated with Bacillus subtilis FQ06 strains and/or rhamnolipid. The optimal solvent extraction conditions were determined as extraction for 20 min at 25 ~C with solvent-soil ratio of 6：1 （v/w）. On this basis, total petroleum hydrocarbon was decreased from 140000 to 14000 mg kg-1, which was further reduced to 〈 4000 mg kg-1 by subsequent bioremediatton for 132 d. Sustainability assessment of this integrated technology showed its good performance for both short- and long-term effectiveness. Overall the results encouraged its application for remediating contaminated sites especially with high concentration weathered hydrocarbons.This study aimed to evaluate the efficacy, practicality and sustainability of a combined approach based on solvent extraction and biodegradation to remediate the soils contaminated with high levels of weathered petroleum hydrocarbons. The soils used in this study were obtained from the Shengli Oilfield in China, which had a long history of contamination with high concentrations of petroleum hydrocarbons. The contaminated soils were washed using a composite organic solvent consisting of hexane and pentane (4:1, v/v) and then bioremediated in microcosms which were bioaugmentated with Bacillus subtilis FQ06 strains and/or rhamnolipid. The optimal solvent extraction conditions were determined as extraction for 20 min at 25 C with solvent-soil ratio of 6:1 (v/w). On this basis, total petroleum hydrocarbon was decreased from 140 000 to 14 000 mg kg-1 , which was further reduced to <4 000 mg kg-1 by subsequent bioremediation for 132 d. Sustainability assessment of this integrated technology showed its good performance for both short- and long-term effectiveness. Overall the results encouraged its application for remediating contaminated sites especially with high concentration weathered hydrocarbons.

关 键 词：Bacillus subtilis BIOAUGMENTATION integrated remediation TU-A solvent rhamnolip 

分 类 号：X53[环境科学与工程—环境工程] TQ126.35[化学工程—无机化工]