2010—2011年深圳市宝安区诺如病毒的分子特征分析  

The molecular characteristics of norovirus gastroenterifis in Baoan district, Shenzhen, 2010-2011

作　　者：张海龙[1] 李苑[2] 姚相杰[1] 冼慧霞[1] 杨洪[1] 罗敏[1] 何雅青[1] 

机构地区：[1]广东深圳深圳市疾病预防控制中心,深圳重大传染病监控重点实验室,518055 [2]深圳市宝安区疾病预防控制中心

出　　处：《国际病毒学杂志》2013年第3期118-122,共5页International Journal of Virology

基　　金：2011年深圳市科技计划项目（201102104）;2011年深圳市宝安区科技创新局资助项目（20110597）

摘　　要：目的了解深圳市宝安区诺如病毒腹泻的分子流行病学特点。方法采集深圳市宝安区2010年1月至2011年12月486例疑似病毒性腹泻患者粪便标本，使用商品化的GⅡ型诺如病毒荧光PCR试剂盒检测GⅡ型诺如病毒核酸。随机挑选若干GⅡ型诺如病毒阳性标本，用GⅡ型诺如病毒特异性引物COG2F／G2-SKR进行RT—PCR扩增，其他所有阴性标本用GI型诺如病毒特异性引物GI—SKF／GI—SKR进行RT—PCR扩增。阳性产物回收纯化并测序，用ClustalW和MEGA5．0生物软件对诺如病毒序列进行序列比对和系统进化分析。结果48份随机挑选的GⅡ型诺如病毒阳性标本中检mGⅡ．4／2006b型36株，GⅡ．4／2008型3株，GⅡ．5型9株；无诺如病毒GⅠ型检出。结论GⅡ型是2010年1月至2011年12月深圳市宝安区诺如病毒的主要型别，其中GⅡ．4／2006b是最重要的流行株。Objective To investigate the molecular characteristics of noroviruses among viral diar- rhea cases in Baoan district, Shenzhen. Methods From January 2010 to December 2011, 486 stool sam- ples were collected from suspected viral diarrhea patients in Baoan district, Shenzhen. Nucleic acid of GII noroviruses were detected using real-time poiymerase chain reaction （real-time PCR）. The positive samples for GII norovirus were randomly selected to perform reverse transcription-polymerase chain reaction （ RT- PCR） with the primers COG2F/G2-SKR, while all the negative samples for GII norovirus were performed RT-PCR with the primers GI-SKF/GI-SKR. Positive PCR products were purified and sequenced, and the se- quences were analyzed by Clustal W and MEGA 5.0 programs, then phylogenetie tree was constructed and genotype was identified. Results Among 48 positive samples for GII norovirus, the numbers of G Ⅱ. 4/ 2006b, G Ⅱ. 4/2008 and GⅡ. 5 were 36, 3, and 9 respectively ; No GⅠ norovirus was found. Conclusions From January 2010 to December 2011, G Ⅱ was the most important genotype of norovirus , and G Ⅱ.4/ 2006b variant was identified as the predominant strain in Baoan district, Shenzhen.

关 键 词：腹泻 诺如病毒 分子流行病学 进化树 

分 类 号：R373[医药卫生—病原生物学]