秦艽花中乙酰胆碱酯酶抑制剂的分离及结构鉴定  

Isolation and Chemical Structure Identification of Acetylcholine Esterase Inhibitor from Gentiana Purdomii

作　　者：党朵[1] 杨中铎[1] 梁冀北[1] 

机构地区：[1]兰州理工大学生命科学与工程学院,甘肃兰州730050

出　　处：《中医药学报》2013年第3期16-18,共3页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金(No.20802031);兰州理工大学优秀青年教师培养计划资助项目(No.Q200904)

摘　　要：目的:从传统中药秦艽花(Gentiana purdomii)中提取分离具有乙酰胆碱酯酶抑制活性的化合物。方法:秦艽花经乙醇提取、酸碱处理、氯仿萃取后得到浸膏,然后经过反复柱层析分离纯化,并结合1H-NMR和13C-NMR谱图分析鉴定单体化合物的结构,最后通过TLC生物自显影测定分离得到的单体化合物对乙酰胆碱酯酶的检测限。结果及结论:共分离得到2个化合物,运用超导核磁共振(NMR)技术,结合文献中已报道的波谱数据等,确定了它们的结构分别为:secostrychnosin(1)、(4R,4aS)-4-vinyl-4,4a,5,6-tetrahydro-3H-pyrano[3,4-c]pyran-1-one(2)。化合物1和化合物2对乙酰胆碱酯酶的最小抑制剂量分别为:0.5μg和1.0μg。Objective：To extracte and isolate acetylcholinesterase inhibitory activity of compound from Gentiana purdomii.Methods：Gentiana purdomii was extracted by ethanol and treated with hydrochloric acid and base,and then extracted by Chloroform to obtain extracts,and the extract was gained and chromatography was separated step by step.Their chemical structures were identified by 1H-NMR and 13C-NMR spectra.The detection limit was extracted by TLC bioautographic assay.Results and Conclusion：Two fractions were obtained by the silica-gel column chromatography.Two compounds were confirmed to be secostrychnosin（1） and（4R,4aS）-4-vinyl-4,4a,5,6-te-trahydro-3H-pyranopyran-1-one（2）.The compounds 1 and 2 of minimal inhibitive dose are 0.5 μg and 1.0 μg.

关 键 词：秦艽花 乙酰胆碱酯酶 抑制剂 化学结构鉴定 最小抑制剂量 

分 类 号：R284.1[医药卫生—中药学]