中国荷斯坦奶牛FoxO1基因多态性及与繁殖性状关系研究  

Polymorphism of FoxO1 gene and associations with reproduction traits in Holstein cattle of China

作　　者：赵佳强[1] 周妍[1] 于倩楠[1] 陶林林[1] 张淑君[1] 易建明[1] 滕茂平 李丛远 张军伟 

机构地区：[1]华中农业大学动物科技学院,农业动物遗传育种与繁殖教育部重点实验室,武汉430070 [2]武汉惠尔康扬子江乳业有限公司,武汉430010

出　　处：《东北农业大学学报》2013年第6期54-57,共4页Journal of Northeast Agricultural University

基　　金：科技部中英重点国际合作项目(2010DFA31820)

摘　　要：通过PCR-单链构象多态性(PCR-SSCP)结合测序技术发现FoxO1第4外显子一个新单核苷酸多态性(SNP),限制性片段长度多态性聚合酶链反应(PCR-RFLP)技术分别分析925头中国荷斯坦奶牛FoxO1该位点多态性。结果表明,在中国荷斯坦奶牛中,FoxO1基因有3个基因型,分别是CC、TT、CT。其中等位基因C频率为0.95,是优势等位基因;χ2适合性检验表明该位点不符合哈代温伯格平衡(P<0.05)。该基因不同基因型对奶牛产犊间隔、空怀期以及初产日龄有显著影响(P<0.05),而对产犊到发情、犊牛初生重、配种次数影响不显著(P>0.05)。PCR-single-strand conformation polymorphism （PCR-SSCP） and sequencing are used and a novel single nucleotide polymorphisms （SNP）was detected in the forth exon of FoxO1 gene. The gene types of the 925 Holsteins were differentiated by PCR-restriction fragment length polymorphism （PCR-RFLP）. The result showed that there were three genotypes （CC, CT, TT） and the frequency of allele C which was superior gene was 0.95; χ2 fitness test showed that the loci deviates from the Hardy-Weinberg equilibrium （P〈0.05）. The correlation analysis revealed that these polymorphisms had a extremely significant effects on calving interval, open days and the first calving age （P〈0.05）, but there was not a significant difference with the days between calving and oestrus, the times of service per conception and birth weight （P〉0.05）.

关 键 词：中国荷斯坦奶牛 SNPS FOXO1 繁殖性状 

分 类 号：S767.5[农业科学—森林保护学] X172[农业科学—林学]