脓毒症对红系造血祖细胞发育的影响  

Effects of sepsis on development of erythroid precursors

作　　者：栗雅杰[1,2] 孔雅娴[1,2] 王蓓蓓[1,2] 苑少欣[1,2] 李彤[1,2] 曾辉[1,2] 张剑平[1,2] 

机构地区：[1]首都医科大学附属北京地坛医院传染病研究所 [2]新发突发传染病研究北京市重点实验室,北京100015

出　　处：《中华实验和临床感染病杂志（电子版）》2013年第2期1-4,共4页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：北京市卫生系统高层次卫生技术人才培养计划(No.2009-2-13)

摘　　要：目的利用小鼠盲肠结扎穿刺模型探讨脓毒症对红系造血祖细胞发育的影响。方法将C57BL/6小鼠分成假手术组和脓毒症组,采用盲肠结扎穿刺术(CLP)建立小鼠脓毒症模型。分别于术后1、3和7d采用流式细胞仪检测小鼠骨髓和脾脏红系造血前体细胞数量和比例的变化。结果与假手术组相比,脓毒症组小鼠骨髓中的巨核细胞-红细胞前体细胞(MEP)比例和数量显著降低;而其脾脏中MEP的比例和数量则有所增加。结论脓毒症模型小鼠骨髓红系造血显著减少,脾脏的红系造血代偿性增加。Objective To investigate the effects of sepsis on development of erythroid precursors with mouse model which received cecal ligation and puncture （CLP）. Methods The mice were divided into two groups. The sepsis model mice built by CLP were taken as sepsis group and sham operation mice were taken as the control group. The changes of red blood cell precursors in bone marrow and spleen were analyzed with flow cytometer at the 1st, 3rd and 7th day after CLP, respectively. Results Compared with the control group, the number and proportion of megakaryocytic-erythroid progenitor cells （MEP） in bone marrow of spesis group decreased significantly. Meanwhile, sepsis mice showed an increased number and frequency of MEP in spleen. Conclusions Erythropoiesis in bone marrow of sespis mice reduced significantly, along with an increase in spleen compensatorily.

关 键 词：脓毒症 红系造血 巨核细胞-红细胞前体细胞 髓外红系造血 

分 类 号：R459.7[医药卫生—急诊医学]