牡蛎过敏原Crag1的二级结构及其B细胞和T细胞表位分析  

Analysis of the secondary structure, B cell and T cell epitopes of oyster allergen Cra g 1

作　　者：伍慧妍[1,2] 何颖[1,2] 许晨霞[1,2] 刘雪婷[1,2] 张俊艳[1,2] 王珊[1,2] 邹泽红[1,2] 陶爱林[1,2] 

机构地区：[1]广州医学院第二附属医院 广州市过敏反应与临床免疫重点实验室,广东广州510260 [2]呼吸疾病国家重点实验室变态反应研究室,广东广州510260

出　　处：《中国医药导报》2013年第19期12-15,19,共5页China Medical Herald

基　　金：国家自然科学基金资助项目(编号30771240);国家转基因生物新品种培育重大专项项目(编号2011ZX08011-005;2009ZX08011-004B);呼吸疾病国家重点实验室(广州医学院)研究课题

摘　　要：目的分析牡蛎过敏原Cra g 1的二级结构及其B、T细胞表位,为探索基于抗原表位的免疫治疗奠定基础。方法从Uniprot数据库中获得牡蛎过敏原Cra g 1的氨基酸序列,通过DNA Star Protean软件,采用Garnier-Robson方法和Chou-Fasman方法分析其二级结构;采用Kyte-Doolittle法、Karplus-Schulz法、Emini法、Jameson-Wolf法综合分析Cra g 1主要的B细胞抗原表位。使用SYFPEITHI和NetMHC-Ⅱ在线网站分析T细胞抗原表位。结果牡蛎过敏原Cra g 1的二级结构主要为α-螺旋,其B细胞表位接近覆盖Cra g 1氨基酸全序列;而T细胞优势表位位于第89～103、108～122、129～143位肽段。结论本研究分析牡蛎过敏原Cra g 1的B、T细胞优势表位,为日后Cra g 1的基础免疫学研究,如疫苗的研制、诊断试剂的制备和其抗原性改造等提供了理论依据。Objective To analyze the secondary structure and B, T cell epitopes of the oyster major allergen Crag 1, develop the immunotherapy of oyster allergy based on antigenic epitopes. Methods The amino acid sequence of oyster major allergen Cra g 1 was acquired from Uniprot database. The secondary structure of Crag 1 was analyzed by DNAStar-Protean software with Gamier-Robson method and Chou-Fasman method. The B cell epitopes of Cra g 1 were analyzed synthetically by Kyte-Doolittle method, Karplus-Schulz method, Emini method and Jameson-Wolf method. The T cell epitopes of Crag 1 were analyzed by SYFPEITHI and NetMHC- Ⅱonline website. Results The analysis of secondary structure showed Cra g 1 was mainly constituted by α-spiral. B cell epitopes covered almost the whole amino acid sequence of Crag 1. However, T cell epitopes analysis demonstrated that the regions of 89-103,108-122 and 129-143 were the dominant T cell epitopes. Conclusion This study, which analyzed the dominant B, T cell epitopes of oyster allergen Cra g 1, provides a theoretical basis for further basic immunology researches of Crag 1,such as vaccine development, diagnosis reagents preparation, and its antigenicity modification.

关 键 词：牡蛎过敏原 CRA g 1 二级结构 B细胞表位 T细胞表位 

分 类 号：R392.8[医药卫生—免疫学]